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《Chinese Journal of Practical Pediatrics》 2008-02
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Vascular endothelial growth factor receptor 2 small hairpin RNA suppresses microvessel angiogenesis in leukemia mice

GUO Hai-xia,CHEN Chun,MAI You-gang,et al.Department of Pediatrics The Second Affiliated Hospital,Sun Yat-Sen University,Guangzhou 510120,China  
Objective In the present study,a lentiviral vector expressing small hairpin RNA against the VEGFR2 gene (Lenti6/sh VEGFR2) was constructed to specifically inhibit VEGFR2 expression and to evaluate the effect of Lenti6/sh VEGFR2 on angiogenesis of HL60 human leukemia cell.Methods Recombine pU6/sh VEGFR2 entry clone with Lenti6/BLOCK-iTTM-DEST vector using LR reaction.The effect of Lenti6/sh VEGFR2 on angiogenesis was evaluated in an HL60 human leukemia cell line.Lenti6/sh VEGFR2 infection of HL60 cells specifically inhibited the VEGFR2 activity as determined by evaluation of the microvessel density of the bone.Results A lentiviral vector expressing small hairpin RNA against the VEGFR2 gene (Lenti6/sh VEGFR2) was constructed.In an HL60 intravenous xenograft leukemia mouse model,Lenti6/sh VEGFR2 infection dramatically suppressed angiogenesis (P 0.05).Conclusion Lentiviral-mediated VEGFR2 RNA interference potentially represents an effective therapeutic strategy against leukemia by inhibiting pathological angiogenesis.
【CateGory Index】: R733.7
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1 GUO Hai-xia1, LI Wen-yi1, XU Ling2, SU Hao-bin1, LI Yang1, XIA Yan1, LIANG Li-yang1(1 Department of Peadiatrics, 2 Center of Medical Research, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China. );Lethal effect of VEGFR_2 shRNA on HL60 cells in vitro using lentiviral vector[J];Chinese Journal of Pathophysiology;2007-06
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1 LIU Yu-bin, LIN Ye, JIAN Zhi-xiang, et al. Depatment of Genaral Surgery, Guangdong Provincial People Hospital, Guangzhou 510080, China;EFFECT OF SOMATOSTATIN ON THE EXPRESSION OF VEGF IN HEPATOCELLULAR CARCINOMA[A];[C];2007
【Secondary Citations】
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1 GUO Hai-xia1,XUE Hong-man1,XIA Yan1,XU Ling2,XU Hong-gui1,LI Wen-yi1(1.Department of Pediatrics,2.Center of Medical Research,The Second Affiliated Hospital,SUN Yat-sen University,Guangzhou 510120,China);Influence of VEGFR2 Expression and Effects on HL60 Cell and Human Endothelial Cell Using siRNA[J];Journal of Sun Yat-Sen University(Medical Sciences);2006-03
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