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《Chinese Journal of Practical Pediatrics》 2008-02
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Vascular endothelial growth factor receptor 2 small hairpin RNA suppresses microvessel angiogenesis in leukemia mice

GUO Hai-xia,CHEN Chun,MAI You-gang,et al.Department of Pediatrics The Second Affiliated Hospital,Sun Yat-Sen University,Guangzhou 510120,China  
Objective In the present study,a lentiviral vector expressing small hairpin RNA against the VEGFR2 gene (Lenti6/sh VEGFR2) was constructed to specifically inhibit VEGFR2 expression and to evaluate the effect of Lenti6/sh VEGFR2 on angiogenesis of HL60 human leukemia cell.Methods Recombine pU6/sh VEGFR2 entry clone with Lenti6/BLOCK-iTTM-DEST vector using LR reaction.The effect of Lenti6/sh VEGFR2 on angiogenesis was evaluated in an HL60 human leukemia cell line.Lenti6/sh VEGFR2 infection of HL60 cells specifically inhibited the VEGFR2 activity as determined by evaluation of the microvessel density of the bone.Results A lentiviral vector expressing small hairpin RNA against the VEGFR2 gene (Lenti6/sh VEGFR2) was constructed.In an HL60 intravenous xenograft leukemia mouse model,Lenti6/sh VEGFR2 infection dramatically suppressed angiogenesis (P 0.05).Conclusion Lentiviral-mediated VEGFR2 RNA interference potentially represents an effective therapeutic strategy against leukemia by inhibiting pathological angiogenesis.
【CateGory Index】: R733.7
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【Citations】
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1 GUO Hai-xia1, LI Wen-yi1, XU Ling2, SU Hao-bin1, LI Yang1, XIA Yan1, LIANG Li-yang1(1 Department of Peadiatrics, 2 Center of Medical Research, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China. );Lethal effect of VEGFR_2 shRNA on HL60 cells in vitro using lentiviral vector[J];Chinese Journal of Pathophysiology;2007-06
【Co-citations】
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1 Jin Donghua, Ye XuezhongDepartment of General Surgery, the Second People's Hospital of Chaohu, Chaohu 238000;The effect of the expression of NF-ΚBp65 with the angiogenesis of hepatocellular carcinoma[J];Anhui Medical Journal;2009-03
2 ZHAO Guo-Guang1,2,XIANG Xiao-Juan1,2,HE You-Jian1,2 1.State Key Laboratory of Oncology in South China, Guangzhou,Guangdong,510060, P.R.China 2.Department of Medical Oncology, Cancer Center, Sun Yat-sen University, Guangzhou,Guangdong,510060, P.R.China;Expression and Clinical Significance of Vascular Endothelial Growth Factor C and D in Nasopharyngeal Carcinoma[J];Chinese Journal of Cancer;2007-01
3 SONG Chang-Cheng, LU Xiang, CHENG Bin-Bin, DU Juan, LI Bai, LING Chang-Quan Department of Traditional Chinese Medicine, Changhai Hospital, Second Militaty Medical University, Shanghai, 200433, P. R. China;Effects of Melittin on Growth and Angiogenesis of Human Hepatocellular Carcinoma BEL-7402 Cell Xenografts in Nude Mice[J];Chinese Journal of Cancer;2007-12
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6 NIU ZHAO-SHAN,JI XIANG-RUI (Lab of Micromorphology,Qingdao University Medical College,Qingdao 266071,China);RELATIONSHIP AMONG INTRATUMOR MICROVESSEL DENSITY,CLINICOPATHOLOGICAL FEATURES AND CELL PROLIFERATION IN HUMAN ENDOMETRIAL ADENOCARCINOMA[J];Acta Academiae Medicinae Qingdao Universitatis;2008-04
7 ZHANG Hai-ying,LI Yan-ru,WANG Jian-jun,LI Xiao-min,WANG Lin(Key Laboratory of Pathobiology,Ministry of Education,School of Basic Medical Sciences,Jilin University,Changchun 130021,China);Establishment of orthotopic transplantation tumor model of liver cancer and its biological features[J];Journal of Jilin University(Medicine Edition);2009-01
8 SHI Wanyi,LIN Fei,HOU Fengjuan(Department of Oncosurgery,the First Affiliated Hospital of Baotou Medical College,Baotou 014010,China);The Expression of C-erbB-2 and Its Correlation to Tumor Angiogenesis in Primary Breast Carcinoma[J];Journal of Baotou Medical College;2007-05
9 ZHANG Shan-Shan, ZHENG Xiang-Rong, YANG Yu-Jia, ZHONG Le, WANG Xia, XIE Min, YU Xiao-He. Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China;Adenovirus-mediated VEGF165 gene transfer has neuroprotective effects in neonatal rats following hypoxic-ischemic brain damage[J];Chinese Journal of Contemporary Pediatrics;2008-06
10 ;膀胱癌能量多普勒血流相对灌注率与微血管密度的相关性研究[J];Contemporary Medicine;2009-09
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1 LIU Yu-bin, LIN Ye, JIAN Zhi-xiang, et al. Depatment of Genaral Surgery, Guangdong Provincial People Hospital, Guangzhou 510080, China;EFFECT OF SOMATOSTATIN ON THE EXPRESSION OF VEGF IN HEPATOCELLULAR CARCINOMA[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 GUO Hai-xia1,XUE Hong-man1,XIA Yan1,XU Ling2,XU Hong-gui1,LI Wen-yi1(1.Department of Pediatrics,2.Center of Medical Research,The Second Affiliated Hospital,SUN Yat-sen University,Guangzhou 510120,China);Influence of VEGFR2 Expression and Effects on HL60 Cell and Human Endothelial Cell Using siRNA[J];Journal of Sun Yat-Sen University(Medical Sciences);2006-03
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