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《Chinese Journal of Practical Pediatrics》 2008-02
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Construction and identification of a cDNA library of infant's small intestine

ZHANG Hui,ZHANG Jing,TIAN Jie,et al.Cardiovascular Department,Children's Hospital,Chongqing Medical University,Chongqing 400014,China  
Objective To construct a cDNA library of infant's small intestine tissue.Methods Total RNA from small intestine tissue was extracted by Trizol.After mRNA purified,cDNA was synthesized by reverse transcription,and cDNA fragments larger than 500bp were ligated with dephosphorylated arms of pUra-MpolyA vectors.The recombination vectors were transformed into DH5α by Cacl2.The size of cDNA inserts were detected by digesting with restriction enzymes EcoRI-XhoI.Results The cDNA library contained 6×107 recombine clones.The insert size ranged from 0.5 to 2.0 kb,averaged 1.5kb.Conclusion A high quality cDNA library from infant's small intestine tissue is constructed successfully which can be used for screening by yeast two-hybrid.
【CateGory Index】: R346
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