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《Chinese Journal of Experimental Traditional Medical Formulae》 2013-16
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HPLC Fingerprint of Shixiao San

ZHOU Xi,CHEN Pei-dong,ZHANG Li,DING An-wei *(Jiangsu Key Laboratory for High Technolog Research of Traditional Chinese Medicine Fomulae,Nanjing University of Chinese Medicine,Nanjing 210046,China)  
Objective: To establish the fingerprint of Shixiao San,enhance the quality control of the method.Method: The RP-HPLC was performed on an C 18 chromatography column,with acetonitrile-0.1% of TFA as mobile phase,the flow rate was 1 mL.min-1;the wavelength was set at 360 nm.The temperature of column was maintained at 30 ℃.Result: The common modes for the HPLC fingerprints were set up by ensuring 15 common peaks in the chromatograms.The similarity degrees in the ten batches were in the range of 0.962 to 0.996.Conclusion: The method is sensitive,accurate and simple,thereby it can be used specifically for the quality control of Shixiao San.
【Fund】: 国家中医药管理局中医药行业科研专项项目(201107007)
【CateGory Index】: R284.1
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