IMMUNOLOGICAL STUDIES ON THE EXTRACTION AND IMMUNOCHEMICAL PROPERTIES OF EXCEOTORY-SECRETORY ANTIGENS OF FASCIOLOPSIS BUSKI
Qian Min Lang So (Department of Biology, East China Normal University, Shanghai 200062)
The excretory-secretory antigens (ES) of Fasciolopsis buski were prcparcd from In vitro cultivation of the adults. The composition of the ES preperations is quite complex. When analy- sed by gel concentration gradient PAGE and SDS-PAGE, it revealed the presence of about 7 protein components and 9 prolypeptide bands. When analysed by immunoelectrophoresis (IE), im- munodiffusion (ID) and enzyme linked immunoelectro-transfer blot (EL/T) of SDS-PAGE, it showed that there were many common antigen components between ES and soluble adult antigens, but only a few between ES and soluble egg antigens.