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《Chinese Journal of Veterinary Parasitology》 2003-01
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ISOLATE HYPODERMOTIN A BY NEGATIVE ION EXCHANGE CHROMATOGRAPHY

XU Meiqian1, GU Yuexing1, SUN Yi1, CAO Jie1, ZHU Shunhai1, MENG Xiuzheng1, HE Guosheng1, N.Moire2, C.Boulard2(1. Shanghai Institute of Domestic Animal Parasitology, CAAS, Shanghai 200232, China; 2.Laboratoire D'immunopathol  
An approach of isolating recombinant Hypodertin(HA), which was secreted into the supernatant of Drosophila expression system(DES), was established by Negative ion exchange chromatography. After the drosophila cells(S2 cell) was cultured for 3 days, the supernatant was collected and concentrated, then dialysed against 25mM pH8.5 TrisHCl buffer. The recombinant HA was isolated by MonoQ column from the dialysed supernatant. The protein peaks were detected at 280nm. SDSPAGE demonstrated that the molecular weight of the first protein was similar to that of natural HA. 0.1% geltin substrate SDSPAGE showed the enzyme activity of recombinant HA. Dotimmunogold verifid the antigen activity. The approach of Negative ion exchange chromatography has many advantages such as easy operation and high efficiency.
【Fund】: 欧盟资助项目:INCO-DEVN0ICA4-CT200030036;;
【CateGory Index】: S858.23
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