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《Journal of Minnan Normal University(Natural Science)》 2018-03
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Electrochemical Studies on the Interaction of a Schiff Base Copper Complex with DNA

NI Jiancong;College of Chemistry, Chemical Engineering and Environment, Minnan Normal University;  
A novel Schiff base copper complex(Cu-SB,SB = Schiff base) was synthesized using o-phenylenediamine,salicylaldehyde and copper acetate as source.The interaction of the Cu-SB with DNA was investigated by electrochemistry and UV spectroscopy.UV spectrum showed that the characteristic absorption peaks of the Cu-SB were hyperchromic effect after the addition of DNA indicating their interaction and the binding constant was determined to be 6.09 ×10~7 M~(-1).It was showed that Cu-SB was an electroactive compound at bare glassy carbon electrode with a pair of irreversible redox peaks.The redox peaks decreased obviously coupling withpositive shift of peak potentials after the addition of DNA, suggesting that Cu-SB might bind with DNA with intercalating mode. Differential pulse voltammetry showed that Cu-SB could be used as an electroactive probe to detect the concentration of DNA in the range of 3.4 ×10~(-5)~1.7 ×10~(-4) M. The surface-based electrochemistry was performed to investigate the kineticparameters between Cu-SB and DNA modified electrode and mechanism of interaction of the two species was further certified.
【Fund】: 福建省自然科学基金面上项目(2018J01436);; 福建省教育厅中青年教师教育科研项目(JAT170344)
【CateGory Index】: O641.4
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