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Effects of Nerve Growth Factor on Neurite Outgrowth and on Synthesis of RNA and DNA in Rat Superior Cervical Ganglia in Vitro: An Autoradiographic Study

Zeng Yuanshan Guo Wanhua (Department of Histology and Embryology, Sun Yat-sen University of Medical Sciences)  
Nerve growth factor (NGF) can promote the outgrowth of neurites of the target ganglia. In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3H-uridine and 3H-thymidine was used. Superior cervical ganglia (SCG) from newborn rats were used for the material of experiments and cultivated by Maximow's double coverslip method. All cultures were divided into a group of NGF (a crude preparation of NGF was added to the medium) and another group served as control (no NGF was added to the medium). Before tissue culture was stopped, the coverslip cultures were transferred to Petri dishes containing the labeling-medium and incubated, and then taken out to be fixated, to be made into serial sections and autoradiographic specimens, which were analysed under the light microscope. The results show that the percentage and the level of grains of neurons uabeled by 3H-uridine in a group of NGF are higher than that of control. Moreover, before the growth rate of neurites shows a peak, the level of grains of neurons labeled by 3H-uridine in a group of NGF is obviously increased. Hence, this evidence suggests that NGF can promote the synthesis of RNA in neurons of SCG, which has a direct bearing on the quick outgrowth of neurites. In the experiments with 3H-thymidine incorporation, that the NGF may promote the synthesis of DNA in some neurons of the third day SCG in vitro was also observed.
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【References】
Chinese Journal Full-text Database 1 Hits
1 Zhou Hancheng Guo Wanhua Zeng Yuanshan Chen Qihui (Department of Histology and Embryology,Sun Yat-Sen University of Medical Sciences,Guangzhou);LOWER DOSE HELIUM-NEON LASER IRRADIATION PROMOTING NEURONAL GROWTH AND RNA SYNTHESIS IN CULTURE[J];Acta Anatomica Sinica;1989-03
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 Lin Meilan, Guo Wanhua (Department of Histology and Embryology, Sun Yat-Sen University of Medical Sciences, Guangzhou);THE NEURITE PROMOTING EFFECTS OF NORMAL AND INJURIED SCIATIC NERVES AND THEIR EXTRACTS ON PC12 CELLS AND SUPERIOR CERVICAL GANGLIA[J];Acta Anatomica Sinica;1990-04
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3 Liu Rengang, Liu Yunhua, Zhu Changgeng Department of Anatomy, Faculty of Basic Medical Sciences;Nigral Cells Transplanted into the Striatum of PD-RatS: Effect of NGF on Grafted Cells[J];Journal of Tongji Medical University;1993-01
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【Co-references】
Chinese Journal Full-text Database 4 Hits
1 Guo Wanhua Zhou Hancheng (Department of Histology and Embryology,Zhongshan Medical College,Guangzhou);CULTIVATION OF SUPERIOR CERVICAL GANGLION OF RAT[J];Acta Anatomica Sinica;1983-04
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【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHU Tai - yong, ZHAO Cui - ping (Henan Luoyang Orthopaedic Institute, Luoyang,Henan471002,China);Experimental Study of Stimulating Effect of Traditional Chinese Drugs on Nerves Regeneration[J];World Journal of Integrated Traditional and Western Medicine;2006-02
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