Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《》 1985-04
Add to Favorite Get Latest Update

Effects of Nerve Growth Factor on Neurite Outgrowth and on Synthesis of RNA and DNA in Rat Superior Cervical Ganglia in Vitro: An Autoradiographic Study

Zeng Yuanshan Guo Wanhua (Department of Histology and Embryology, Sun Yat-sen University of Medical Sciences)  
Nerve growth factor (NGF) can promote the outgrowth of neurites of the target ganglia. In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3H-uridine and 3H-thymidine was used. Superior cervical ganglia (SCG) from newborn rats were used for the material of experiments and cultivated by Maximow's double coverslip method. All cultures were divided into a group of NGF (a crude preparation of NGF was added to the medium) and another group served as control (no NGF was added to the medium). Before tissue culture was stopped, the coverslip cultures were transferred to Petri dishes containing the labeling-medium and incubated, and then taken out to be fixated, to be made into serial sections and autoradiographic specimens, which were analysed under the light microscope. The results show that the percentage and the level of grains of neurons uabeled by 3H-uridine in a group of NGF are higher than that of control. Moreover, before the growth rate of neurites shows a peak, the level of grains of neurons labeled by 3H-uridine in a group of NGF is obviously increased. Hence, this evidence suggests that NGF can promote the synthesis of RNA in neurons of SCG, which has a direct bearing on the quick outgrowth of neurites. In the experiments with 3H-thymidine incorporation, that the NGF may promote the synthesis of DNA in some neurons of the third day SCG in vitro was also observed.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 Zhou Hancheng Guo Wanhua Zeng Yuanshan Chen Qihui (Department of Histology and Embryology,Sun Yat-Sen University of Medical Sciences,Guangzhou);LOWER DOSE HELIUM-NEON LASER IRRADIATION PROMOTING NEURONAL GROWTH AND RNA SYNTHESIS IN CULTURE[J];Acta Anatomica Sinica;1989-03
Chinese Journal Full-text Database 5 Hits
1 Lin Meilan, Guo Wanhua (Department of Histology and Embryology, Sun Yat-Sen University of Medical Sciences, Guangzhou);THE NEURITE PROMOTING EFFECTS OF NORMAL AND INJURIED SCIATIC NERVES AND THEIR EXTRACTS ON PC12 CELLS AND SUPERIOR CERVICAL GANGLIA[J];Acta Anatomica Sinica;1990-04
2 Xiong Renping, Zhou Yuanguo, Zhu Peifang, Zhou Jihong, Liu DaweiResearch Institute of Surgery, Third Military Medical College of PLA (Chongqing 630042);Isolation of the Nerve Growth Factor from Male Rat Maxillary Glands[J];Chinese Journal of Biochemical Pharmaceutics;1996-03
3 Liu Rengang, Liu Yunhua, Zhu Changgeng Department of Anatomy, Faculty of Basic Medical Sciences;Nigral Cells Transplanted into the Striatum of PD-RatS: Effect of NGF on Grafted Cells[J];Journal of Tongji Medical University;1993-01
4 Ren Wanqiong ,Feng Jun, Liu Baoshan,Song Guoming,Zhou Wenli (Biological and Medical Products Research Institue of Jinan Military Area,Zhumadian 463000);Preparation of Freeze-Dry Mice Nerve Growth Factor on A Medium Scale[J];PHARMACEUTICAL BIOTECHNOLOGY;2000-01
5 Luo Huanmin,Yao Zhibin, Xie Yao et alBrain Lab,SUMS, Guangzhou 510089;Effects of modified Dangguishaoyaosan(mDSS) on cultured hippocampal neurons[J];CHINESE JOURNAL OF GERONTOLOGY;1998-02
Chinese Journal Full-text Database 4 Hits
1 Guo Wanhua Zhou Hancheng (Department of Histology and Embryology,Zhongshan Medical College,Guangzhou);CULTIVATION OF SUPERIOR CERVICAL GANGLION OF RAT[J];Acta Anatomica Sinica;1983-04
2 Huang Lianbi, Yang Shuzhen, Xu Jing(Department of Histology and Embryology, Zhongshan Medical College);CHARACTERISTICS OF MITOSIS OF NERVE CELLS IN THE CEREBRAL CORTEX OF ADULT MAMMALS AFTER TRAUMA[J];Acta Anatomica Sinica;1984-01
3 Guo Wanhua, Zhou Hancheng, Chen Oihui, Zheng Shusen~* (Department of Histology and Embryology, Zhongshan Medical University, Guangzhou) Laboratory of Electron Microscope, Zhongshan Medical University Guangzhou;DISSOCIATED CELL CULTURE FROM SYMPATHETIC GANGLIA AND OBSERVATIONS WITH SCANNING ELECTRON MICROSCOPE[J];Acta Anatomica Sinica;1986-01
4 Guo Wanhua Ramon Lim~* (Department of Histology and Embryology,Sun Yat-Sen University of Medical Sciences,Guangzhou.~*Department of Neurology,Division of Neurochemistry and Neurobiology,University of Iowa College of Medicine,Iowa City,USA);GLIA MATURATION FACTOR SUPPORTS THE SURVIVAL OF CEREBELLAR CORTICAL NEURONS IN PRIMARY CULTURE[J];Acta Anatomica Sinica;1989-01
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHU Tai - yong, ZHAO Cui - ping (Henan Luoyang Orthopaedic Institute, Luoyang,Henan471002,China);Experimental Study of Stimulating Effect of Traditional Chinese Drugs on Nerves Regeneration[J];World Journal of Integrated Traditional and Western Medicine;2006-02
2 Zhu Tai-yong Wang Jian-zhi Luoyang Institute of TCM Orthopedics and Traumatology,Henan(471002);Experimental Study on Promoting Effects of Modified Buyang Huanwu Tang(加味补阳还五汤)Injection on Ganglion Neurite's Growth in Vitro[J];Chinese Journal of Integrated Traditional and Western Medicine;1995-S1
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved