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《中山医科大学学报》 1992-02
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RANDOM PRIMERED LABELLING OF HBV DNA AND ITS APPLICATION IN REHYBRIDIZATION

Lu Ling Yao Jilu Peng Wenwei (Department of Infectious Diseases, 3rd Affiliated Hospital)  
The method of random primered labelling was used to prepare 32P labelled HBV DNA probe. By liquid scintillation, its specific activity was tested to be 109 cpm/μg, incorporation rate of 12P 71.43%, both were higher than those observed in nick translation. By applying it in 5ng (6×106 cpm) in 5 ml per 100 cm2 membrane to detect HBV DNA spotted on nylon filter, the probe was found to have a sensitivity up to 0.1pg and a positivity rate as high as 87%(26/30 )in both HBsAg and HBeAg carrier sera, after the filter was exposed to X-ray film for 24 hours. Digoxigenin labelled HBV DNA probe was also prepared by this technique and applied in 20μg/L(100 ng in 5 ml per 100 cm2 membrane) to rehybridize HBV DNA detected by 32P labelled probe mention above.It gave a sensitivity up to 0.05Pg and a positivity rate as high as 73%(22/30).
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【References】
Chinese Journal Full-text Database 2 Hits
1 $$$$ Lu Ling;Yao Jilu;Peng Wenwei;Huang Yangsu (Department of Infectious Diseases The 3rd Affiliated];AMPLIFICATION AND DETERMINATION OF HEPATITIS B VIRUS SEQUENCESIN SERUM POSITIVE TO ANTI-HBc AND THE NICK TRANSLATION TO GENERATE DNA PROBE[J];ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;1994-01
2 Gao Zhiliang;Yao Jilu (Department of Infectious Diseases , The 3rd Affiliated Hospital , Sun Yat-sen University of Medical Sciences, Guangzhou , 510630);APPLICATION OF A NEW TECHNIQUE FORSIMULTANEOUSLY AMPLIFYING WITH HBVDNA AND HCV RNA IN SERUM[J];ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;1996-02
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Lu Ling Xu Lin Peng Wenwei Yao Jilu Zheng Xicheng ( Hepatitis Unit, Sun Yat-Sen University of Medical Sciences );PREPARATION AND APPLICATION OF DIGOXIGENIN- LABELLED HBV DNA PROBE[J];;1991-02
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Lu Hongyan Yao Jilu Lu Ling (Department of Infectious Disease);DETECTION OF HEPATITIS B VIRUS SEQUENCES BY NESTED POLYMERASE CHAIN REACTION[J];;1992-03
2 Gan Zhiliang Yao Jilu Lu Ling Huang Yangsu Zheng Xicheng (Department of Infectious Diseases, the Third Affiliated Hospital);A SIMPLIFIED TECHNIQUE OF HBVDNA ISOLATION IN PCR[J];;1993-03
3 $$$$ Gao Zhiliang; Yao Jilu;DIRECT AMPLIFICATION OF HCV RNA GENOME[J];ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;1994-03
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Xie Yanbo Ma Binnan Lu Ling Peng Wenwei (Hepatitis Research Laboratory, Molecular Biology Research Laboratory);Preparation of Biotinated HBV DNA Probe and Its Application in Liver Diseases[J];;1987-03
2 Lu Ling Peng Wenwei He Shuchu Xie Yanbo (Department of Infectious Diseases, Third Affiliated Hospital );INTEGRATION OF HEPATITIS B VIRUS DNA IN HEPATOCELLULAR CARCINOMA[J];;1990-02
3 Lu Ling Peng Wenwei Ho Shuchu Xie Yanbo (Department of Infectious Diseases, Third Affiliated Hospital);INFECTION OF HEPATITIS B VIRUS IN HEPATOCELLULAR CARCINOMA: AN ANALYSIS OF HBV DNA IN SERUM AND LIVER[J];;1990-03
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