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《Journal of Sun Yat-sen University (Medical Sciences)》 2005-01
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Establishment of Stromal Cell Lines of Human Aorta-gonad-mesonephros (AGM) Region and Its Biological Characteristics

CHEN Hui-qin, ZHANG Xu-chao, HUANG Shao-liang, WEI Jing, WU Bei-yan(Center for Stem Cell Research, The Second Affiliated Hospital, SUN Yat-sen University, Guangzhou 510120, China)  
To establish stromal cell lines of human aorta-gonad-mesonephros (AGM) region and study the growth characteristics and hematopoiesis related molecules expression in these cells. Stromal cells of AGM region in human embryos of day 30-35 were isolated and cultured in vitro. Besides their growth characteristics were studied, hematopoiesis related molecules including surface and extracellular matrix proteins were analyzed respectively by flow cytometry and immunofluorescence methods. Stromal cells of human AGM region held the morphology of fibroblastoid cells. During logarithmic growth period, the cell population doubling time is about 16 h. By flow cytometry, surface markers CD31, CD34, CD45 were negatively expressed, while CD29, CD44, CD105, CD166 were positively detected on human AGM stromal cells. Through immunofluorescence assay, hematopoiesis related extracellular proteins such as tenascin, fibronectin, laminin and α-SMA were all positively expressed by stromal cells of AGM region. [Conclusion] These stromal cells of human AGM region have the characteristics of early blood-forming tissue specific molecules expression, and can be used as model cells for further hematopoiesis mechanisms research.
【Fund】: 国家自然科学基金(30300377);; 教育部博士点基金(20030558070);; 中国博士后科学基金资助项目(20003033432)
【CateGory Index】: R329.2
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