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《Journal of Sun Yat-sen University (Medical Sciences)》 2005-01
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Proteins with Altered Expression Induced by Epstein-Barr Virus in Nasopharyngeal Carcinoma CNE2 Cells

JIANG Pei-zhou 1, 2, GAN Ming 3, HUANG Hua 1, SHEN Xin-ming 1, YING Wan-tao 4, QIAN Xiao-hong 4, YAO Kai-tai 1(1. Cancer Research Institute, The First Military Medical University, Guangzhou 510515, China; 2. Department of Histology and Embryology, The First Military Medical University, Guangzhou 510515, China; 3. Department of Parasitology, Medical College, SUN Yat-sen University, Guangzhou 510080, China; 4. Beijing Institute of Radiation Medicine, Beijing 100850, China)  
To seek and identify functional protein with altered expression in CNE2 cells after Epstein-Barr virus (EBV) infection and then provide clues to clarify the mechanism of biological changes induced by EBV in nasopharyngeal epithelial cells.The total protein from experimental group-CNE2 cells infected with EBV and control group-CNE2 cells without EBV infection was extracted and then separated by two-dimensional electrophoresis (2-DE) and Coomassie brilliant blue staining respectively. Subsequently, the protein expression patterns of the above two groups were compared and analyzed with software to seek protein spots altered in expression. Then the protein spots were excised from gels and identified by the matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). Image analysis revealed that there were some obviously different protein spots between experimental group and control group. Four protein spots of them were successfully identified by MALDI-TOF-MS. The result showed that GRP78 and thioredoxin peroxidase 1 were up-regulated while actin cytoplasmic 2 and proteasome iota chain were down-regulated in CNE2 cells after EBV infection. [Conclusion] The results suggest that the molecular mechanism of biological changes in nasopharyngeal epithelial cells induced by EBV might be as follows: (1) EBV might induce cells to synthesize amounts of protein for proliferation, and up-regulate the expression of protein GRP78 to antagonize apoptosis.(2)EBV might increase the oxidation in cells so as to induce the expression of protein "thioredoxin peroxidase 1", and then regulate the expression of NF-κB and AP-1 via thioredoxin peroxidase 1 to affect cell growth.
【Fund】: 国家86.3科研基金资助项目(2001AA216101);; 国家973科研基金资助项目(2001CB5102080)
【CateGory Index】: R739.63
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