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《Journal of Sun Yat-Sen University(Medical Sciences)》 2008-01
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Eukaryotic Expression of Renal Cell Carcinoma G250 Antigen Gene in sp2/0 Cells and Its Significance

ZHANG Ya-dong1,WU Rong-pei1, LIU Lei1, LI Le-jun1, CAO Kai-yuan2, LI Xiao-fei1( 1. Department of Urology, The First Affiliated Hospital;2. The Standard Clinical Examination Center, SUN Yat-sen University, Guangzhou 510080, China )  
[Objective] To provide a tool for the therapy of gene vaccine based on G250 gene, we transferred it into sp2/0 cells to obtain stable expression. [Methods] The constructed recombinant plasmid was transfected into sp2/0 cells, which were screened by G418, then the positive cells was identified. [Results] Two weeks after transfection, we got positive cells. RT-PCR and indirect immunofluorescence methods showed that the G250 gene was expressed in positive sp2/0 cells. [Conclusion] The cell model expressing G250 antigen was established, which provided a basis for further exploring the therapy of gene vaccine based on RCC G250 gene.
【Fund】: 广东省科技攻关项目(2003c31201);; 广州市科技攻关项目(2003Z2-E4011)
【CateGory Index】: R737.11
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