Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Biological Characteristics in Cervical Cancer Cell Line Hela Tolerated to Radiation

HUANG Jing-ran,PENG Yong-pai,ZHOU Hui,FENG Min-qing,YAO Ting-ting, RAO Qun-xian,WANG Li-juan,LIN Zhong-qiu(Department of Gynecology Oncology,Sun Yet-Sen Memorial Hospital,Sun Yet-Sen University,Guangzhou 510120,China)  
【Object】 The aim of the study was to investigate biological behaviors of radioresistant cervical cancer cells.The relationship between radioresistant cancer cells and cancer stem cells would be discussed.【Methods】 Hela cells were treated with fractionated irradiation,yielding Hela-R,the radioresistant model.There were four groups: Hela-R1,Hela-R2,Hela-R3 and control.Biological characteristics,including cell growth,clone-generating capability,cell cycle distribution and proliferation,and tumor sphere-forming rate were detected by MTT assay,clone formation assay,flow cytometry assay and serum-free medium culturing,respectively.【Results】 Before growth inhibition,both the parental cells and the sub-lines Hela-R were accelerated proliferation by exposure to irradiation.In the three groups of Hela-R,cell doubling times were(43.4 ± 1.0)h,(49.2 ± 2.0)h,and(48.7 ± 3.3)h;the colony forming rates were(20.3 ± 4.0)%,(49.3 ± 11.6)%,and(6.3 ± 5.9)%;S-Phase Fraction were(17.6 ± 1.3)%,(20.0 ± 2.4)%,and(23.9 ± 2.3)%;Proliferation index(PI) were(27.3 ± 2.6)%,(31.8 ± 4.9)%,and(37.4 ± 8.0)%,respectively.Compared with the parental cells,cells in Hela-R3 were more radioresistant to electronic rays.The formation of tumor spheres could be observed when Hela cells and Hela-R cells were cultured in non-adherent serum-free medium,and tumor sphere-forming rates were(9.9 ± 0.4)%,(13.0 ± 0.9)%,(9.6 ± 0.7)%,and(5.0 ± 0.3)%,respectively.【Conclusion】Long-term fractionated radiation exposure conferred tolerance of ionizing radiation to the human tumor cells Hela,which is a method to establish radioresistant model and obtain tumor stem cells by radiation enrichment.Hela-R cells were more powerful in self-renew and cloning-generating capability,with a trend of proliferative capability increased,while the cellular growth was slower and cell cycle showed no significant change.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved