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《Journal of Sun Yat-sen University(Medical Sciences)》 2016-01
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Up-regulation of miR-100 Attenuates Invasiveness, Migration and Cis-platin Resistance in Cervical Cancer Cells

LIU Yun-yun;LU Huai-wu;LI Jing;WANG Dong-yan;LI Rui-xin;ZHOU Hui;LIN Zhong-qiu;Department of Gynecological Oncology,Sun Yet-San Memorial Hospital;  
【Objective】 To observe the invasiveness, migration and cis-platin resistance in cervical cancer cell lines Hela and Siha up-regulated with mir-100 mimics. 【Method】The expression of mir-100 was up-regulated using mimics via lipomax. Cells were grouped and labeled with ‘NC' and ‘mir100'. Transwell assay and scarification test were used to reveal the invasiveness and migration of cervical cancer cells, western blot was used to test protein expressions, cck-8 was used to test resistance to cis-platin. 【Result】With the up-regulation of mir-100, Hela and Siha lost invasiveness and migration; E-Cadherin increases while snail and vimintin decrease after mir-100 transfection, MMP2 showed no change and u PA was attanuted. Hela and Siha cells were much more sensitive to cis-platin therapy after up-regulation of mir-100. 【Conclusion】 mi R-100 attanuates EMT in cervical cancer cell Hela and Siha. Upregulation of mi R-100 decreases resistance to cis-platin in Hela and Siha.
【Fund】: 教育部博士点基金(20120171110097);; 卫生部临床重点基金(2010439)
【CateGory Index】: R737.33
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