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《Journal of Sun Yat-sen University(Medical Sciences)》 2016-06
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Hepatic Stellate Cells Inhibit Proliferation of T Cells through Induction of CD8~+CD28~-T Lymphocytes

LIU Qiu-li;LIU Chang;YANG Fan;ZHENG Hai-qing;Cell-gene Therapy Translational Medicine Research Center ,The Third Affiliated Hospital of Sun Yat-sen University;Cell-gene Therapy Translational Medicine Research Center,The Third Affiliated Hospital of Sun Yat-sen University;Department of Rehabilitation Medicine,The Third Affiliated Hospital of Sun Yat-sen University;  
【Objective】To investigate whether hepatic stellate cells(HSC) inhibit the proliferation of T cells through the induction of CD8~+CD28-T lymphocytes. 【Methods】CD3~+T cells were sorted by flow cytometry and labeled by 5,6-carboxyfluoresceindiacetate,succinimidyl ester(CFSE), then they were co-cultured with human hepatic stellate cells cell line LX2 in the present of PHA for72 h. The proliferation of T cells was evaluated by flowcytometry.In order to study the mechanism of the inhibition of T cells by hepatic stellate cells, purified CD3~+T cells were co-cultured with LX2 for 3 days, and the percentage of CD8~+CD28-T cells was detected by flowcytometry. Furthermore, after CD3~+T cells were co-cultured with LX2 cells for 3 days, CD8~+CD28-T cells were sorted by flow cytometry. Then purified CD8~+CD28-T cells were co-cultured with CFSE labeled CD3~+T cells, and the proliferation of T cells was evaluated after stimulated with PHA for 72 h. 【Results】T cell proliferation was inhibited obviously after co-cultured with LX2 cells.The inhibitory effect was dose dependent. LX2 cells dose-dependently increased the percentage of CD8~+CD28-T cells. CD8~+CD28-T cells, which cultured with LX2 cells, obviously inhibited the proliferation of T cells. 【Conclusion】HSC inhibited the T cell proliferation through the induction of CD8~+CD28-T regulatory cells.
【Fund】: 国家自然科学基金(81572228 81601381);; 广东省自然科学基金(2015A030313015 2016A030310131);; 中山大学高校青年教师培育项目(15ykpy26);; 广东省科技计划项目(2014A020212715)
【CateGory Index】: R575
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【Citations】
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1 Ching-Chuan Hsieh;Chien-Hui Hung;Lina Lu;Shiguang Qian;Department of Surgery, Chang Gung Memorial Hospital at Chiayi;Graduate Institute of Clinical Medical Sciences, Chang Gung University;Department of Immunology, Lerner Research Institute, Transplantation Center, Digestive Disease Institute, Cleveland Clinic;Department of General Surgery,Transplantation Center, Digestive Disease Institute, Cleveland Clinic;;Hepatic immune tolerance induced by hepatic stellate cells[J];World Journal of Gastroenterology;2015-42
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