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《Journal of Sun Yat-sen University(Medical Sciences)》 2016-06
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Effects of IL-22 on Apoptosis of Rheumatoid Arthritis Fibroblast-like Synoviocytes

LIU Meng;LIU Yan;YANG Meng-ru;MO Bi-yao;PAN Yun-feng;Department of Rheumatoid and Immunology,The Third Affiliated Hospital of Sun Yat-sen University;  
【Objective】To investigate the effect of interleukin(IL)-22 on the apoptosis of rheumatoid arthritis fibroblast-like synoviocytes(RA-FLS). 【Methods】Synovium tissues collected and isolated from patients who had joint replacement surgery or arthroscopy, which were cultured by tissue culture method. Bcl-2 m RNA and Bcl-XL m RNA expression in FLS(RA, Osteoarthritis,Trauma) were determined by q RT-PCR.Bcl-2 protein expression in FLS were detected by Western blotting. RA-FLS were treated with nitric oxide(NO) donor sodium nitroprusside(SNP) in the present or absence of rh IL-22, RA-FLS apoptosis was analyzed by flow cytometry. Effects of different concentrations of rh IL-22(0, 1, 10, 100 ng / m L) on the expression of Bcl-2 protein level in RAFLS and OA-FLS were detected by Western Blotting. 【Results】The Bcl-2 m RNA(P 0.05), Bcl-XL m RNA(P 0.01) and Bcl-2 protein(P0.01) were increased in RA-FLS compared with OA-FLS and Trauma-FLS. Flow cytometry assay showed rh IL-22 did not affect RA-FLS apoptosis compared with control group(P 0.05), SNP induced RA-FLS apoptosis obviously(P 0.01),rh IL-22 prevented RA-FLS apoptosis in the presence of SNP(P 0.01). rh IL-22 could up-regulate the expression of Bcl-2 in both RA-FLS and OA-FLS(P 0.05), but RA-FLS were more sensitive to rh IL-22. 【Conclusion】IL-22 can protect RA-FLS from SNP-induced apoptosis, at least in partly by up-regulating the expression of Bcl-2.
【Fund】: 广东省自然科学基金项目(2014A030313080)
【CateGory Index】: R593.22
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【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 GUO Xin1,HU Ai-ling2,FANG Lin-kai1,LIU Yan1,PAN Yun-feng1(1Department of Rheumatology,2Department of Nursing,the Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China);Effect of RICTOR expression on cell viability in rheumatoid arthritis fibroblast-like synoviocytes[J];中国病理生理杂志;2013-03
2 GUO Xing-hua1,PAN Yun-feng1,SONG Ze-rong1,WANG Kun2,GU Jie-ruo1(1Department of Rheumatology,2Department of Orthopedics,The Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China.);Effects of fractalkine on nuclear factor-κB activation and endogenous fractalkine mRNA expression in fibroblast-like synoviocytes from patients with rheumatoid arthritis[J];中国病理生理杂志;2011-10
3 XIAO Chu-yin,PAN Yun-feng,GUO Xing-hua,WU Yu-qiong,HUANG Jian-lin,GU Jie-ruo;Effects of lithium chloride on cell proliferation of fibroblast-like synoviocytes[J];中国病理生理杂志;2010-08
4 YANG Chunhua,HUANG Feng.Department of Rheumatology,General Hospital of PLA,Beijing 100853, China;Expression of urokinase type plasminogen activator and its receptor in rheumatoid arthritis[J];中华风湿病学杂志;2000-03
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