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《Journal of Sun Yat-sen University(Medical Sciences)》 2016-06
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Effects of IL-22 on Apoptosis of Rheumatoid Arthritis Fibroblast-like Synoviocytes

LIU Meng;LIU Yan;YANG Meng-ru;MO Bi-yao;PAN Yun-feng;Department of Rheumatoid and Immunology,The Third Affiliated Hospital of Sun Yat-sen University;  
【Objective】To investigate the effect of interleukin(IL)-22 on the apoptosis of rheumatoid arthritis fibroblast-like synoviocytes(RA-FLS). 【Methods】Synovium tissues collected and isolated from patients who had joint replacement surgery or arthroscopy, which were cultured by tissue culture method. Bcl-2 m RNA and Bcl-XL m RNA expression in FLS(RA, Osteoarthritis,Trauma) were determined by q RT-PCR.Bcl-2 protein expression in FLS were detected by Western blotting. RA-FLS were treated with nitric oxide(NO) donor sodium nitroprusside(SNP) in the present or absence of rh IL-22, RA-FLS apoptosis was analyzed by flow cytometry. Effects of different concentrations of rh IL-22(0, 1, 10, 100 ng / m L) on the expression of Bcl-2 protein level in RAFLS and OA-FLS were detected by Western Blotting. 【Results】The Bcl-2 m RNA(P 0.05), Bcl-XL m RNA(P 0.01) and Bcl-2 protein(P0.01) were increased in RA-FLS compared with OA-FLS and Trauma-FLS. Flow cytometry assay showed rh IL-22 did not affect RA-FLS apoptosis compared with control group(P 0.05), SNP induced RA-FLS apoptosis obviously(P 0.01),rh IL-22 prevented RA-FLS apoptosis in the presence of SNP(P 0.01). rh IL-22 could up-regulate the expression of Bcl-2 in both RA-FLS and OA-FLS(P 0.05), but RA-FLS were more sensitive to rh IL-22. 【Conclusion】IL-22 can protect RA-FLS from SNP-induced apoptosis, at least in partly by up-regulating the expression of Bcl-2.
【Fund】: 广东省自然科学基金项目(2014A030313080)
【CateGory Index】: R593.22
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 LIU Yan;PAN Yun-feng;FANG Lin-kai;GUO Xin;WU Yun-ting;Department of Rheumatology and Immunology,The Third Affiliated Hospital of Sun Yat-sen University;Department of Rheumatology and Immunology,The Second People's Hospital of Guangdong Province;;Effects and Mechanisms of uPAR Expression in Rheumatoid Arthritis Fibroblast-like Synoviocytes[J];中山大学学报(医学科学版);2014-02
2 Lian-Zhen Yu, Hai-Yang Wang, Shu-Ping Yang, Zhi-Ping Yuan, Fang-Yuan Xu, Chao Sun, Rui-Hua Shi, Department of Gastroenterology,First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China , Department of Internal Medicine, Affiliated Mingde Hospital of Nanjing Medical University, Nanjing 211166, Jiangsu Province, China;Expression of interleukin-22/STAT3 signaling pathway in ulcerative colitis and related carcinogenesis[J];World Journal of Gastroenterology;2013-17
3 GUO Xin1,HU Ai-ling2,FANG Lin-kai1,LIU Yan1,PAN Yun-feng1(1Department of Rheumatology,2Department of Nursing,the Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China);Effect of RICTOR expression on cell viability in rheumatoid arthritis fibroblast-like synoviocytes[J];中国病理生理杂志;2013-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XIN Ke-bei;ZHANG Jian-li;YANG Juan;BIAN Xiao-ping;WEI Hui-na;Department of Gastroenterology, Affiliated hospital of Zhengzhou, jinan university, The Second People's Hospital of Zhengzhou;;A Study on the Effect of Tanshinone ⅡA Sulfonate Injection on the Clinical Efficacy of Ulcerative Colitis[J];现代生物医学进展;2015-34
2 ZHANG Chenfei;ZHAO Guangyao;YU Zhuwen;DAI Juan;ZHU Lanxiang;CHEN Weichang;Department of Gastroenterology,the First Affiliated Hospital of Soochow University;Department of Gastroenterology,the First People’s Hospital of Changzhou;;Expressions and Significance of Interleukin-22,Matrix Metalloproteinase-9 and Macrophage Migration Inhibitory Factor in Peripheral Blood of Patients with Inflammatory Bowel Disease[J];胃肠病学;2015-07
3 WANG Qiang;GUO Dezheng;LIU Yanwei;SHI Jia;HUANG Jian;HAO Langsong;Guizhou Medical University;Department of General Surgery,Guizhou Provincial People's Hospital;Department of General Surgery,Lu'an Municipal Hospital;Department of General Surgery,Taihe Hospital;;The Prognostic Value of HIF-1α Combined with STAT3 for Early Predicting the Heterochrony CRLM in Colorectal Cancer[J];贵阳医学院学报;2015-07
4 Yong-Hong Huang;Yun-Fei Cao;Zhi-Yuan Jiang;Sen Zhang;Feng Gao;Department of Colorectal and Anal Surgery, First Affiliated Hospital of Guangxi Medical University;;Th22 cell accumulation is associated with colorectal cancer development[J];World Journal of Gastroenterology;2015-14
5 LI Xiaodong;ZHANG Xueyan;ZHENG Lei;LI Xiaolin;Department of Gastroenterology,the Second Affiliated Hospital to Harbin Medical University;;The role of interleukin in ulcerative colitis[J];胃肠病学和肝病学杂志;2015-01
6 WANG Qian;ZOU Jian;ZHANG Xiu-fen;MU Hui-jun;YIN Ying;XIE Ping;Central Laboratory,The Affiliated Wuxi People's Hospital,Nanjing Medical University;;Glucosylceramide synthase upregulates apoptosis-related gene bcl-2 expression via MEK/ERK signaling pathway in leukemia multidrug-resistant cell line[J];中国病理生理杂志;2015-01
7 Guo Xin;Pan Yunfeng;Fang Linkai;Guo Xinghua;Liu Yan;Wu Yunting;Department of Rheumatology,Guangdong No.2 Provincial People's Hospital;;Role of RICTOR on the tumor necrosis factor-α associated cell activation in rheumatoid arthritis fibrobast-like synoviocytes[J];中华风湿病学杂志;2015-01
8 REN Jie;ZHOU Yi;WU Huixia;ZHU Lihua;DAI Taoli;Department of Rheumatology, First Affiliated Hospital of Jinan University;Medical Biotechnology Research and Development Center of Jinan University;;NKp44~+NK cells induce STAT3 signal pathway-dependent RA FLS proliferation by producing IL-22[J];免疫学杂志;2015-01
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10 Lin Guo;Chang-Ping Li;Department of Gastroenterology,the Affiliated Hospital of Luzhou Medical College;;Mechanisms underlying therapeutic effects of curcumin on ulcerative colitis[J];世界华人消化杂志;2014-20
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 GUO Xin1,HU Ai-ling2,FANG Lin-kai1,LIU Yan1,PAN Yun-feng1(1Department of Rheumatology,2Department of Nursing,the Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China);Effect of RICTOR expression on cell viability in rheumatoid arthritis fibroblast-like synoviocytes[J];中国病理生理杂志;2013-03
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