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《Journal of Sun Yat-sen University(Medical Sciences)》 2016-06
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miRNA-21 and miRNA-134 as Potential Serum Markers in Diagnosis of Breast Cancer

SHI Yan;QIN Yan;SONG Lei;LIANG Yue-mian;WANG Xiao-lei;HAN Gui-liang;Department of Medical Oncology//Key Laboratory for Fractionation Mechanisms and Procedures of Heibei,Affiliated Hospital of Hebei University;Department of Chinese Radiology, Affiliated Hospital of Hebei University;Department of Central Lab, Affiliated Hospital of Hebei University;Department of Pathology, Affiliated Hospital of Hebei University;  
【Objective】Exploring the expression of breast cancer related miRNA in the serum of normal population and breast cancer patients, analysis its clinical value in the diagnosis of breast cancer. 【Methods】Serumswere collected from normal population and breast cancer patients. RT-PCR was used to test the expression of miR-630, miR-21, miR-195, miR-134, miR-200 a, miR-381 and miR-1228.The value of single serum miRNA in diagnosis of breast cancer wereanalyzed, the diagnostic value of combined detection of multiple miRNA were further analyzedby using statistical methods. 【Results】Compared to normal population, serum miR134 and miR21 were increased in breast cancer patients, P 0.01; the expression of miR-134 had no difference between the early stage breast cancer patients and late stage breast cancer patients, P = 0.143; the expression of miR-21 increased in the late stage breast cancer patients, P = 0.004. Meanwhile, the expression of miR-195, miR-630, miR-200 a, miR-381, miR-1228 levels between the two groups had nostatistical significance. ROC curve analysis showed that, in the diagnosis of breast, AUC value of miR-21 was 0.82(95% CI, 0.71-0.88), P 0.001; AUC value of miR-134 was 0.88(95% CI, 0.80-0.94), P 0.001; AUC value of miR-21 combined with miR-134 in the diagnosis of breast was 0.95(95% CI, 0.89-0.99), P 0.001, which was better than a singledetector miR-21 or miR-134, P 0.05. 【Conclusion】The expression of serum miR-21 and miR-134 in the breast cancer patients was elevated and miR-21 combined with miR-134 can be used as an ideal marker for the diagnosis of breast cancer.
【Fund】: 河北大学附属医院青年科研基金(2015Q014)
【CateGory Index】: R737.9
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