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《Journal of Sun Yat-sen University(Medical Sciences)》 2017-01
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IL-4 Activated Macrophages Promote Hela Cell Migration and Invasion by Producing CCL18

HAN Jie;LI Shun-ying;LI Qian;LIU Yu-jie;Department of Gynecological Oncology,Shanghai eighth People's hospital;Breast Tumor Center,Sun Yat-sen Memorial Hospital;  
【Objective】To investigate the roles of IL-4 activated macrophages on Hela cells migration and invasion and the underlying mechanism.【Methods】The phenotype of IL-4 activated macrophage was assayed by morphology,flow cytometry and Elisa. The ability of Hela cell migration and invasion was evaluated by transwell assay. The signaling proteins expression was displayed by Western blot.【Results】Il-4 activated macrophage had the phenotype of CD206high/HLA-DRlow,and highly expressed IL-10 and CCL18,and decreased the expression of IL-12. The activated macrophages significantly promoted the migration and invasion of Hela cells,while the unactivated macrophages did not had this effect. Applying anti-CCL18 antibody but not isotype Ig G in the supernatant of IL-4 activated macrophage inhibited Hela cells motility. Furthermore,activated macrophages phosphorylated IKK and IκB and induced the translocation of p65 to nucleus,eventually increased the expression of vimentin. Treatment of Hela cells with CCL18 also enhanced tumor cells migration and invasion,as well as the NF- κB activation and vimentin expression.【Conclusion】IL-4 activated macrophages promoted Hela cells motility by secreting CCL18 and the following activation of NF- κB signaling proteins.
【Fund】: 高校基本科研业务费中山大学青年教师培育项目(14ykpy20);; 高等学校博士学科点专项科研基金(新教师类)(20110171120082)
【CateGory Index】: R737.33
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【References】
Chinese Journal Full-text Database 1 Hits
1 Haoyue Guo;Rui Mao;Ran Wang;Erjia Zhu;Donglai Chen;Chang Chen;Department of Thoracic Surgery,Tongji University School of Medicine;Shanghai Pulmonary Hospital,Tongji University School of Medicine;;Role of different phenotype tumor-associated macrophages in tumor progression[J];中国肿瘤临床;2018-09
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 HAN Jie;LI Shun-ying;LI Qian;LIU Yu-jie;Department of Gynecological Oncology,Shanghai eighth People's hospital;Breast Tumor Center,Sun Yat-sen Memorial Hospital;;IL-4 Activated Macrophages Promote Hela Cell Migration and Invasion by Producing CCL18[J];中山大学学报(医学科学版);2017-01
2 Chen Liwen;Zhou Qiang;Zhong Fei;Wang Qin;Fang Yaping;Yang Kai;Guan Shihe;Clinical Laboratory,the Second Hospital of Anhui Medical University;;Expression of CC-chemokine ligand 18(CCL18) in the serum and pleural effusion of non-small-cell lung cancer patients and its regulatory effect on the differentiation of monocyte-derived dendritic cells[J];中华肿瘤杂志;2014-11
3 CHEN Jingqi;ZENG Bohang;ZHU Bisheng;HOU Kailian;Department of Oncology, No.2 Affiliated Hospital, Guangzhou Medical College;;CCL18 Promotes Migration of Breast Cancer Cells via Binding to Its Functional Membrane Receptor[J];肿瘤防治研究;2014-02
4 Wang Ruili1,Zhang Ting1,Ma Zhengwen2,et al.1.Department of Obstetrics and Gynecology,Renji Hospital,Shanghai Jiaotong University,School of Medicine,Shanghai 200001;2.Department of Neurobiology,Shanghai Jiaotong University,School of Medicine;Coagulation factor Ⅻ induced MO differentiation into a TAM like macrophage subset in ovarian cancer[J];现代妇产科进展;2010-04
5 ZHANG Bicheng1,ZHAO Yong1,WANG Jun2,GUO Yan2,RAO Zhiguo1,GAO Jianfei11Dept.of Oncology,Wuhan General Hospital,Guangzhou Command,People's Liberation Army,Wuhan 430070,China2 Dept.of Oncology,General Hospital of Jinan Command,People's Liberation Army,Jinan 250031,China;Activated Phenotypes Identification of Tumor-Associated Macrophages in Human Lung Adenocarcinoma[J];武汉大学学报(医学版);2009-05
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