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《ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES》 1997-01
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CONSTRUCTION OF RECOMBINED VACCINIA VIRUS EXPRESSING HUMAN CEA

Yang Jie 1 Yang Taicheng 2 Luo Chaoquan 1 Wang Xiaohuai 2 Yang Yinghao 1 Jiang Yuehua 2 Wu Xinyao 1 (1 Department of Biochemistry, Sun Yat sen University of Medical Sciences, Guangzhou, 510089 2 General Hospital of PLA of Guangzhou  
By using vaccinia virus as a vector, we have successfully established a CEA vaccinia virus recombinant capable of highly expressing CEA. After infecting 143TK cells with the recombinant, the expressed products could be detected by radio immunoassay technique only in cell membrane fragment but not in cell plasma and culture supernate. It further proved that vaccinia virus is an efficient vector for expressing eukaryotic protein and can maintain the characteristics of the protein. Based on this result, it is possible to study whether CEA vaccinia virus recombinant can activate immuno reactive system in vivo for combatting against tumors expressing CEA.
【Fund】: 广东省卫生厅“九五”五个一工程项目资助
【CateGory Index】: Q344.13
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【References】
Chinese Journal Full-text Database 3 Hits
1 Lu Fangan; Huang Bing;Yang Jie et al(Department of Biochemistry, Yunyang medical College.Shiyan442000);The Establishment of An in Vitro Experimental Model of Murine Hepa Cancer cells Transferred by Recombinant Human CEA Plasmid[J];JOURNAL OF YUNYANG MEDICAL COLLEGE;1999-04
2 Lu Fangan,Yangjie,Luo Chaoquan,et al (Department of Biochemistry,Yunyang Medical College,Shiyan 442000);Construction of CEA-cDNA EukaryoticExpression Recombinant Plasmid[J];JOURNAL OF YUNYANG MEDICAL COLLEGE;1999-02
3 Li Yungui Wang Xiaohuai Yang Taicheng et al Department of Oncology,Guangzhou Sanatorium of PLA,Guangzhou;Construction and Expression of Carcinoembryonic Antigen Recombinant Plasmids with Two Different Promoters[J];Chinese Journal of Clinical Oncology;2003-08
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 YANG Jie,LUO Chaoquan,LU Fangan,YANG Yinghao,WU Xinyao (Sun Yat Sen University of Medical Sciences,Guangzhou 510089);Construction and Production of CEA recombinant Vaccinia Virus[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1999-01
2 Lei Dianliang,Ma Xiao,Gu Lei, et al.(National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050);An MTT colorimetric assay for measurement of the activity of hepatic stimulating substances[J];PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY;1999-03
3 Wang Qi, Wang Bo, Bai Huiqing (Dept of Microbiology of Ningxia Medical College);Examination of the Killing Activity of NK Cells with Modified MTT Approach[J];JOURNAL OF NINGXIA MEDICAL COLLEGE;1999-02
4 Wang Yanping;Cheng Xiaohe;Zhang Wengeng;et al. (Center of Cancer,the First Hospital,West China University of Medical Sciences,Chengdu 610041);The detection of LAK cell activity using SMMC-7721 cells as the target cells with MTT colorimetry[J];CHINESE JOURNAL OF CLINKAL ONCOLOGY AND REHABILITATION;1999-06
5 He Jinsheng;Li Ruizhu;Zong Tingyi( Department of Microbiology,Anhui Medical University,Hefei 230032);INVESTIGATION OF NK CELL ACTIVITY MEASUREMENT[J];CHINESE JOURNAL OF IMMUNOLOGY;1996-06
6 Yang Jie 1 Lai Huangwen 2 Luo Chaoquan 1 Yang Taichang 2 Xu Guoheng 1 Wang Xiaohuai 2 (1 Department of Biochemistry,Sun Yat sen University of Medical Sciences,Guangzhou,510089; 2 Department of Medical Experiment,General Army Hospital,;STUDY OF THE CEA cDNA RECOMBINANT VACCINIA VIRUS TO VACCINE ANIMALS[J];ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;1997-S1
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 LI Yun-gui, ZHANG Wei-jie,WANG Xiao-huai,et al. Department of Oncology, Guangzhou Sanatorium, PLA, Guangzhou Guangdong 510515, China;Effect of Different Promoters on CEA Expression and Immune Responses Elicited by Carcinoembryonic Antigen DNA Vaccine in Mice[J];Military Medical Journal of South China;2005-01
2 ZHOU Hua min, WANG Jun, SU Yong quan, DING Shao xiong, YAN Qing pi, ZHANG Zhao xia(Dept. of Oceanog. and Instit. of Subtropical Oceanog., Xiamen Univ., Xiamen 361005, China);Construction of Eukaryotic Expression Recombinant Plasmid of Fla I Gene from Vibrio parahaemolyticus[J];Journal of Xiamen University(Natural Science);2002-04
3 CHEN Rong-ming , YING Min-gang , ZHENG Qiu-hong , et al.(Department of Tumor Molecular Biology , Fujian Provincial Tumor Hospital , Fuzhou 350014,China);Creation of Eukaryotic Expression Vector for Human Carcinoembryonic Antigen (CEA) Gene[J];Bulletin of Chinese Cancer;2005-02
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