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Yang Jie 1 Yang Taicheng 2 Luo Chaoquan 1 Wang Xiaohuai 2 Yang Yinghao 1 Jiang Yuehua 2 Wu Xinyao 1 (1 Department of Biochemistry, Sun Yat sen University of Medical Sciences, Guangzhou, 510089 2 General Hospital of PLA of Guangzhou  
By using vaccinia virus as a vector, we have successfully established a CEA vaccinia virus recombinant capable of highly expressing CEA. After infecting 143TK cells with the recombinant, the expressed products could be detected by radio immunoassay technique only in cell membrane fragment but not in cell plasma and culture supernate. It further proved that vaccinia virus is an efficient vector for expressing eukaryotic protein and can maintain the characteristics of the protein. Based on this result, it is possible to study whether CEA vaccinia virus recombinant can activate immuno reactive system in vivo for combatting against tumors expressing CEA.
【Fund】: 广东省卫生厅“九五”五个一工程项目资助
【CateGory Index】: Q344.13
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Chinese Journal Full-text Database 3 Hits
1 Lu Fangan; Huang Bing;Yang Jie et al(Department of Biochemistry, Yunyang medical College.Shiyan442000);The Establishment of An in Vitro Experimental Model of Murine Hepa Cancer cells Transferred by Recombinant Human CEA Plasmid[J];JOURNAL OF YUNYANG MEDICAL COLLEGE;1999-04
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Chinese Journal Full-text Database 6 Hits
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【Secondary References】
Chinese Journal Full-text Database 3 Hits
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