EFFECTS OF PEROXOVANADATE NICOTINIC ACID ON PHOSPHOENOLPYRUYATE CARBOXYKINASE ACTIVITY IN DIABETIC RATS
Wang Yanlin Yu Binjie Yuan Minsheng Zhang Yijian Xiao Yibin (Department of Endocrinology, The First Affiliated Hospital of Sun Yat sen University of Medical Sciences, Guangzhou, 510089)
Liver phosphoenolpyruvate carboxykinase (PEPCK) activity of rat with diabetes treated by peroxovanadate nicotinic acid (POV) and metformin was determined by spectrophotometrically enzyme coupling rate method. The results show liver PEPCK activity of diabetic rat's was increased significantly, compared with that of normal rats ( P 0.01). The rat's PEPCK activity was inhibited obviously after treated with POV for 4 weeks. The enzymatic activity of the diabetic rats treated with metformin was unobviously affected on PEPCK activity of normal rats. The results provided theoretic basis for the molecular biochemical mechanism of POV lowering hyperglycemia, and provided experimental base for exploring a new lowering hyperglycemia drug.