IMMUNOHISTOCHEMICAL DETECTION OF HCV INFECTION IN PATIENTS WITH HEPATOCELLULAR CARCINOMA AND OTHER LIVER DISEASES
Zhang Lifa Pen Wenwei Yao Jilu (Department of Infectious Diseases, Third Affiliated Hospital, Sun Yat sen University of Medical Sciences, Guangzhou, 510630)
Using labelled streptoavidin biotin (LSAB) method with monoclonal antibody to NS3 region of HCV genome, we detected the expression of HCV antigen in the liver tissues of different liver deseases (HCC, 52 cases; cirrhosis, 16 cases; chronic hepatitis, 40 cases; constitutional jaundice, 8 cases) and 6 normal liver samples as negative control. The results showed that in the three groups of HCC, cirrhosis and chronic hepatitis, the positive rates of HCV antigen were respectively 13.5%(7/52), 12.5%(2/16), and 10%(4/40), while in the samples from patients with constitutional jaundice and normal liver samples, no HCV antigen was found. HCV antigen could be seen in the nuclei or/and cytoplasms of carcinoma cells and/or pericancerous hepatocytes. The results supported the view that HCV is associated with HCC and HCV infection enhances the development of liver diseases. In HCC, HCV antigen was more often seen in nuclei than in cytoplasms. The positive rate of HCV antigen in pericancerous tissues was higher than in cancerous tissues, and this result indicated that HCV affects the initiative period of HCC and induces the malignant phenotypic alteration of hepatocytes.