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《Chinese Journal of Veterinary Medicine》 2004-01
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Cloning and prokaryotic expression of NS1 gene from goose parvovirus

WANG Jun-wei~1,LI Meng~1,MA Bo~1,BU Ru-e~1,LIU Bao-quan~1,Ulrich Neumann~2(1.College of Veterinary Medicine, Northeast Agricultual University, Harbin 150030, China; 2.Clinic for Poultry, Hannover School of Veterinary Medicine, Hannover D-30545, Germany)  
According to the published sequence of GPV B strain in GenBank, a pair of primers was designed by Oligo4.0. NS1 gene of GPV was amplified by PCR. The completegene was cloned into the vector PMD18-T and sequenced. The result of sequence showed that NS1 gene comprised of 1884 its could able to encode 627 amnio acids. The cloned NS1 gene shared 98.15% homology with the B strain. Meanwhile,the NS1 gene was inserted in the BamHⅠ site of plasmid PGEX-6P-1 to construct prokaryotic expression vector of GPV NS1 gene,which expressed 97 kD fusion protein of NS1.
【Fund】: 黑龙江“十五”攻关课题 ( GB0 1B5 0 3 -0 2 )
【CateGory Index】: S852.65
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