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《Chinese Journal of Veterinary Medicine》 2012-01
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Immunoprotection of recombinant protein Vp1 against duck hepatitis virus MY strain

LU Jiao,LUO Wei,LIU Nei-sheng,LIU Qun (College of Life Science&Biotechnology,Southwest University for Nationalities,Chengdu 610041,China)  
Prokaryotic expression vector(pET32a+Vp1,BL21(DE3)PlysS) was employed to express the Vp1 gene of duck hepatitis virus MY strain.The purified recombinant protein was used as antigen to produce subunit vaccine.And then ducklings were inoculated with the subunit vaccine,and the indirect ELISA was used to determine the level of antibody.Meanwhile,challenge test was performed to evaluate the effect of immunoprotection.The results showed that after rabbits were immunized by the Vp1,the agar gel precipitation titer in serum was more than 1:64,and neutralization titer was 1/45 After ducklings were immunized,increasing tendency of antibody was observed,which could prevent ducklings from duck hepatitis virus challenge.This study revealed the ability of Vp1 expressed in organism and prevent ducklings from duck hepatitis virus for the first time.
【Fund】: 四川省科技厅应用基础研究“鸭肝炎鸡胚化弱毒MY株的生物学特性及其疫苗生产条件的研究”(2008JY0069)
【CateGory Index】: S858.32
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