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《Chinese Journal of Veterinary Medicine》 2012-01
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Immunoprotection of recombinant protein Vp1 against duck hepatitis virus MY strain

LU Jiao,LUO Wei,LIU Nei-sheng,LIU Qun (College of Life Science&Biotechnology,Southwest University for Nationalities,Chengdu 610041,China)  
Prokaryotic expression vector(pET32a+Vp1,BL21(DE3)PlysS) was employed to express the Vp1 gene of duck hepatitis virus MY strain.The purified recombinant protein was used as antigen to produce subunit vaccine.And then ducklings were inoculated with the subunit vaccine,and the indirect ELISA was used to determine the level of antibody.Meanwhile,challenge test was performed to evaluate the effect of immunoprotection.The results showed that after rabbits were immunized by the Vp1,the agar gel precipitation titer in serum was more than 1:64,and neutralization titer was 1/45 After ducklings were immunized,increasing tendency of antibody was observed,which could prevent ducklings from duck hepatitis virus challenge.This study revealed the ability of Vp1 expressed in organism and prevent ducklings from duck hepatitis virus for the first time.
【Fund】: 四川省科技厅应用基础研究“鸭肝炎鸡胚化弱毒MY株的生物学特性及其疫苗生产条件的研究”(2008JY0069)
【CateGory Index】: S858.32
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Chinese Journal Full-text Database 5 Hits
1 XIANG Yi-yong1,LUO Wei1,JIN Yan-ling2,LIU Nei-sheng1,LIU Qun1,LONG Hu1(1.College of Life Science and Biotechnology,Southwest University for Nationalities,Chengdu 610041,China;2.Chengdu Institute of Biology,Chinese Academy of Science,Chengdu 610041,China);Cloning and Prokaryotic Expression of Chicken-embryo-adapted Attenuated Vaccine Strain MY of Duck Hepatitis Virus and Antigenicity of the Expressed Protein[J];China Animal Husbandry & Veterinary Medicine;2010-12
2 LUO Wei1, ZHANG Jin-ling2, LIU Nei-sheng1, PEI Chao-xin3YUE Hua1, YANG Xiao-nong1(1.College of Life Science&Biotechnology,Southwest University for Nationalities,Chengdu 610041,China;2.Sichuan Animal Science Academy,Chengdu 610041,China;3.Sichuan Animal Disease Control and Prevention Center,China);BREED AND TRIAL ON CHICKEN EMBRYO ATTENUATED STRAIN MY OF DUCK HEPATITIS VIRUSⅠ[J];Journal of Inner Mongolia Agricultural University(Natural Science Edition);2010-01
3 CAO Su-fang,HUANG Qing-yun (South China Agricultural University,Guangzhou 510642,China; College of Zhengzhou Animal Husbandry Engineering,Zhengzhou 450011, China);Studies on immune efficacy of recombinant subunit vaccine with mature outer membrane protein H against avian Pasteurella multocida C_(48-1)[J];Veterinary Science in China;2006-06
4 LIU Jia-sen1,2,3,GAN Yi-di3,JIANG Qian3,MENG Qing-wen3,HAN Ling-xia3,SI Chang-de3,LIU Di1,QU Lian-dong1,2,3(1.Post-doctoral Station of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;2.Post-doctoral Station of Northeast Forestry University,Harbin 150040,China;3.National Key Laboratory of Veterinary Biotechnology/Laboratory Animal Center,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China);Cloning and prokaryotic expression of VP3 gene of duck hepatitis virus typeⅠ[J];Chinese Veterinary Science;2008-07
5 HE Ran-ya,YU Miao,ZHANG Yu-ling,ZHANG De-yu,CAO Zong-xi,ZHANG Gui-hong (College of Veterinary Medicine,South China Agricultural University,Guangdong 510642,China );EPIDEMIOLOGICAL INVESTIGATION AND GENETIC VARIATION IN VP1 GENE OF DUCK HEPATITIS VIRUS ISOLATES FROM IN SOUTHWESTERN CHINA IN 2007-2009[J];Chinese Journal of Animal Infectious Diseases;2010-01
Chinese Journal Full-text Database 10 Hits
1 LIAO Jun-wei1,2,ZHANG Xiao-fei2,3,HUANG Xian-ming1,2,MAO Huo-yun1,2,YIN Xiu-feng2,LIU Da-wei1,2,WEI Jian-zhong1 (1.College of Animal Science and Technology,Anhui Agriculture University,Hefei,Anhui,230036,China 2.Nanjing Tech-Bank Bio-industry Co.,LTD,Nanjing,Jiangsu,211102,China 3.Veterinary Research Institute of Jiangsu Academy of Agricaltural Sciences,Nanjing,Jiangsu,210014,China);Cloning and Prokaryotic Expression of VP3 Gene of Duck hepatitis virus Type Ⅰ[J];Progress in Veterinary Medicine;2009-07
2 ZHANG Yan-fang,LUO Wei,LIU Nei-sheng,LIU Qun(College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China);Advance of Duck Hepatitis Virus[J];China Animal Husbandry & Veterinary Medicine;2011-07
3 HUANG Liang-zong1,LIU Man-yi2,WANG Shu-min1,SI Xing-kui1,GU Wan-jun1(1.Department of Animal Medicine,Foshan University,Foshan 528231,China; 2.Epidemic Diseases Prevention and Quarantine of Nanshan,Foshan 528000,China);Molecular cloning and sequence analysis of complete genome of DHV-Ⅰ SN strain and attenuated vaccine strain[J];Guangdong Agricultural Sciences;2011-12
4 HUANG Liang-zong1,LIU Man-yi2,WANG Shu-min1,SI Xing-Kui1,GU Wang-jun1(1.Department of Animal Medicine,Foshan University,Foshan,Guangdong,528231,China;2.Epidemic Diseases Prevention and Quarantine of Nanshan,Foshan,Guangdong,528000,China);Biological Characteristics and Sequence Analysis of Polyprotein Gene of Duck Hepatitis Virus GD-B Strain[J];Progress in Veterinary Medicine;2011-11
5 HE Cui,ZHANG Lei,LI Ke,Entomack Borrathybay,Wulumuhan Nazierbieke(College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China);Cloning and Expression of omp H Gene of Avian Pasteurella Multocida Strain X-73[J];Journal of Jishou University(Natural Sciences Edition);2009-06
6 ZHANG Jing,DONG Jia-wen,LUO Yong-wen,GUO Xiao-feng(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China);Construction of Recombinant Duck Plague Virus with VP1 Gene of Duck Hepatitis Virus Type Ⅰ[J];Journal of South China Agricultural University;2011-04
7 Wulumuhan Nazierbieke1,Fang Yan1,Cui He1,Lei Zhang1,and Entomack Borrathybay1,2 1 Laboratory of Bioengineering,College of Biology and Environmental Sciences,Jishou University,Jishou 41600,China 2 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Xinjiang University,Urumuqi 830046,China;Expression and Purification of an Adhesive Protein of Rabbit Pasteurella multocida C51-3 and Detection of Its Antigenicity[J];Chinese Journal of Biotechnology;2008-08
8 NAZIERBIEKE Wulumuhan1 CHEN Liang2 OMAR Kamalibieke3 YAN Fang1BORRATHYBAY Entomack1,2(1 College of Biology and Environmental Sciences,Jishou University,Jishou 416000,China)(2 College of Life Science and Technology,Xinjian University,Urumuqi 830046,China)(3 College of Chemistry and Chemical Engineering,Xinjiang University,Urumuqi 830046,China);Prokaryotic Expression and Purification of a Mature Adhesive Protein of Avian Pasteurella multocida P1059 and Detection of Its Antigenicity[J];China Biotechnology;2008-04
9 Ulumuhan Nazierbieke1,PENG Qing-Zhong1,HE Cui1,ZHANG Lei1,YAN Fang1,Entomack Borrathybay1,2 1.Laboratory of Bioengineering,College of Biology and Environmental Sciences,Jishou University,Jishou 41600; 2.Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Xinjiang University,Urumuqi 830046;China;Cloning and Sequencing of a Mature Adhesive Protein Gene cpm39 of Avian Pasteurella multocida C_(48-3)[J];Letters in Biotechnology;2008-01
10 GONG Qiang,WANG Shuai-Tao,QIN Cui-Li,NIU Ming-Fu,CHENG Ming,HOU Yu-Ze,ZHANG Yong-Fa,SUN Xiao-Fei.Henan University of Science & Technology,Luoyang 471003,China;The immune efficacy of OmpA-DNA vaccine against avian pasteurella multocida[J];Chinese Journal of Immunology;2011-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Ping, Pan Wen-shi, Hu Shou-wen Department of Biology;Duck virus hepatitis in Peking duckling I. Diagnosis and prevention[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1980-01
2 XU Cheng,CHEN Ya-bo,FANG Wei-huan (Institute of Preventive Veterinary Medicine of Zhejiang University and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine,Hangzhou,Zhejiang,310029,China);Prokaryotic Expression of Structural Protein VP1 of Foot-and-mouth disease virus Type O and Analysis of Its Antigenicity[J];Progress in Veterinary Medicine;2007-06
3 ZHAO Rui-hong1,ZHANG Xiao-fei2,3,WEN Li-bin2,PAN Xiao-cheng1(1.Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Hefei 230031,China;2.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;3.Nanjing Tech-bank Bio-industry Co Ltd,Nanjing 211102,China);Construction and Analysis of Partial cDNA Library of Duck Hepatitis Virus Strain A_(66)[J];China Animal Husbandry & Veterinary Medicine;2007-09
4 QIAO Chuan ling 1,2 ,JIANG Yong ping 1,2 ,LI Cheng jun 1,2 , TIAN Guo bin 2,MENG Qing wen 1,2 , DENG Guo hua 1,2 , WANG Xiu ro ng 1,2 , YU Kang zhen 1,2 ,TANG Xiu ying 2(1.National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Resea rch Institute, CAAS, Harbin 150001,China; 2. Center of Animal Influenza,Harbin 150001, China);Studies on live fowlpox virus vector-based vaccine of avian influenza rFPV-HA-NA[J];Immunological Journal;2003-01
5 WU Pei-fu,HAN Bo,ZHANG Guo-zhong,LIU Jin-hua,SU Jing-liang (College of Veterinary Medicine,China Agricultural University,Beijing 100193,China);Sequencing and molecular analysis of duck hepatitis virus type 1,strain F[J];Journal of China Agricultural University;2008-06
6 Cheng Anchun et al(College of Animal Science and Technology, SichuanAgricultural University/Yaan, Sichuan, China 625014);STUDY ON BIVALENT ATTENUATED VACCINES AGAINST DP AND DVH Ⅱ、THE DETECTION OF THE STABILITY, THE CONDITION AND THE PERIOD OF THE BIVALENT ATTENUATED VACCINES PRESERVED[J];Journal of Sichuan Agricultural University;1996-01
7 ZHANG Zhen-hua1,2,ZHOU Xue-mei2,4,CHEN Nan-hui1,CHEN Xiao-ling2,LUO Chang-bao3,LI Yong-qing2 1.Department of Veterinary Medicine,Jiangxi Agricultural University,Nanchang 330045,China;2.Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100089,China;3.Guangzhou Bureau of Entry-Exit Inspection and Quarantine,Guangzhou 510623,China;4.College of Animal Medicine,China Agricultural University,Beijing 100094,China;Cloning and Structural Analysis of Chicken Complement Component 3d Gene[J];Letters in Biotechnology;2006-04
8 Xiuli Ma1,2,Minxun Song2,Kexiang Yu2,Ming Liao1,Chao’an Xin1 (1South China Agricultural University,Guangzhou 510642,China) (2Shandong Academy of Agricultural Sciences,Ji’nan 250023,China);Expression of VP1 gene and ELISA detection of antibodies against duck hepatitis virus[J];Acta Microbiologica Sinica;2008-08
9 Shaohua Shi 1,2,Longfei Cheng 1,2,Guanghua Fu1,Hongmei Chen 1,2,Zhen Chen1,Weixing Yang3,Jingliang Su 4,Yu Huang 1 (1 Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)(2 Fujian Animal Diseases Control Technology Development Center,Fuzhou 350013,China)(3 College of animal science and technology,Jiangxi Agricultural University,Nanchang 330045,China)(4 College of Veterinary Medicine,China Agricultural University,Beijing 100094,China);Genomic sequence of a new serotype duck hepatitis virus[J];Acta Microbiologica Sinica;2009-03
10 WANG Li xin, XU Wei, GUAN Qing dong, XIONG Si dong Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University; Center for Gene Immunization and Vaccine Research, Shanghai 200032, China;Enhancement of HBV gene-induced specific cell-mediated immunoresponse by C3d-P28[J];Journal of Cellular and Molecular Immunology;2003-03
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