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《Chinese Journal of Veterinary Medicine》 2012-02
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Establishment of a Cycling probe real-time PCR assay for Brucella detection

XU Zou-liang1,2,NAN Wen-long1,LU Ming-zhe1,ZHOU Jie1,TAN Peng-fei1,2, CHEN Yi-ping1,MAO Kai-rong3(1.Diagnostic Reagent Laboratory,China Animal Health and Epidemiology Center,Qingdao 266032 China; 2.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China; 3.China Institute of Veterinary Drug Control,Beijing 100081,China)  
A Cycling real-time PCR assay was developed for the detection of all species and bivors of Brucella.According to the sequences of BCSP31 gene,a probe and two primers were designed.The sensitivity and standard curve of the Cycling real-time PCR assay were tested.All results showed that the detection limit of the assay was about 10 copies of the recombinant plasmid and the standard curve was favorable with 0.999 linear correlation coefficient.The specificity was perfect with typical "S" curve amplification of Brucella and negative results for other four irrelevant bacterias.Furthermore,the detection of 40 clinical samples showed 35 positive samples were detected by the Cycling probe real-time PCR,which included all 27 B4/B5-PCR positive samples.The coincidence rate of two methods was 80.0%.All results suggested that the Cycling probe real-time PCR assay was specific,sensitive and stable,which could be used to diagnose clinical samples.
【Fund】: “十一五”国家支撑计划(2006BAD06A12);; 公益性行业(农业)项目(200903027)
【CateGory Index】: S855.12
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【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 ZHANG Hongyan JIANG Wenguo YAN Bingxin 1.Maternal and Child Health Care Hospital of Shizhong District,Jining 272100,China;2.Center for Disease Control and Prevention of Jining City,Jining 272100,China;Risk Factors for Human Infection of Brucellosis Case-control Study[J];China Medicine and Pharmacy;2011-06
2 LI Ye,YU Xiao-hua,HE Tian-feng The Base for Plague and Brucellosis Control and Prevention,Chinese Center for Disease Control and Prevention, Jilin Baicheng 137000,China;An analysis of brucellosis epidemic situation in humans from 2004 to 2010 in China[J];Chinese Journal of Control of Endemic Diseases;2012-01
3 ZHU Su-juan,XU Wei-min,WANG Heng,YANG Yang,JIN Hang-yi,TANG Yi,WANG Jia.Hangzhou Center for Disease Control and Prevention,Hangzhou 310021,Zhejiang,China;Surveillance of human brucellosis in Zhejiang province,2005-2009[J];Disease Surveillance;2011-02
4 BAI Yong-fei,XU Li-hong,GUO Zhi-xi,TIE Ping,YAN Chang-fu,ZHAO Liang-huai.Shanxi Provincial Center for Disease Control and Prevention,Taiyuan 030012,Shanxi,China;Time series Autoregressive Integrated Moving Average model to predict brucellosis incidence in Shanxi province[J];Disease Surveillance;2011-08
5 JIANG Wenguo YAN Bingxin Center for Disease Control and Prevention of Jining City, Jining 272000,China;Analysis on Epidemiological Characteristics and Epidemic Factor of Brucellosis in Jining City[J];China Modern Doctor;2010-32
6 XU Chang-xi,LU Xin-rong,PENG Yue-hua Department of Infectious Diseases Control and Prevention,Center for Disease Control and Prevention of Jilin Province,Changchun Jilin 130062,China;Analysis of epidemic situation of human brucellosis in Jilin province during 1990-2010[J];Chinese Journal of Public Health Engineering;2012-01
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