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《Chinese Journal of Veterinary Medicine》 2012-02
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Expression of Jilin white goose interferon-α in Escherichia coli and detection for its antiviral activity

LI Gong-mei1,LI Yu-mei2,HU Jing-tao1,LIU Ke-yue3,QIAN Ai-dong1 (1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China; 2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China; 3.Jiujiang University Medical College,Jiujiang 332000,China)  
The interferon αgene of JiLin White goose was amplified from genomic DNA,the segment coding mature peptide of the GoIFN-α gene was cloned into prokaryotic expression vector pGEX-6p-1 to construct recombinant plasmid pGEX-IFN-α.pGEX-IFN-αwas transformed into E.coli BL-21(DE3) for the expression of fused protein GST-IFN-α in E.coli BL-21(DE3) by IPTG induction.The expressed product was identified by SDS-PAGE and Western-blot.The results showed that fusion protein GST-IFN-αwas about 45ku and it could react with interferon antibodies.After purification with Glutathione Sepharose-4B affinity column,the activity of recombinant GoIFN-α was detected by inhibiting the cytopathic effect in fibroblast cells infected with Goose parvovirus.The result showed that the recombinant GoIFN-α has bicactivity and its activity against Goose parvovirus(GPV) at the duck embryo fibroblast cell was 3.32×105 U/mg.
【Fund】: 吉林省科技发展计划项目(201101112);; 吉林省教育厅“十二五”科学技术研究项目(吉教科合字[2011]第49号)
【CateGory Index】: S852.612
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