Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Veterinary Medicine》 2012-02
Add to Favorite Get Latest Update

Expression of Jilin white goose interferon-α in Escherichia coli and detection for its antiviral activity

LI Gong-mei1,LI Yu-mei2,HU Jing-tao1,LIU Ke-yue3,QIAN Ai-dong1 (1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China; 2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China; 3.Jiujiang University Medical College,Jiujiang 332000,China)  
The interferon αgene of JiLin White goose was amplified from genomic DNA,the segment coding mature peptide of the GoIFN-α gene was cloned into prokaryotic expression vector pGEX-6p-1 to construct recombinant plasmid pGEX-IFN-α.pGEX-IFN-αwas transformed into E.coli BL-21(DE3) for the expression of fused protein GST-IFN-α in E.coli BL-21(DE3) by IPTG induction.The expressed product was identified by SDS-PAGE and Western-blot.The results showed that fusion protein GST-IFN-αwas about 45ku and it could react with interferon antibodies.After purification with Glutathione Sepharose-4B affinity column,the activity of recombinant GoIFN-α was detected by inhibiting the cytopathic effect in fibroblast cells infected with Goose parvovirus.The result showed that the recombinant GoIFN-α has bicactivity and its activity against Goose parvovirus(GPV) at the duck embryo fibroblast cell was 3.32×105 U/mg.
【Fund】: 吉林省科技发展计划项目(201101112);; 吉林省教育厅“十二五”科学技术研究项目(吉教科合字[2011]第49号)
【CateGory Index】: S852.612
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Liu Changnuan, Liu Lan, Wang Junzhi et al(National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050);A Colorimetric Method for Determination of IFN Potency[J];CHINESE JOURNAL OF BIOLOGICALS;1999-01
2 Xia Chun,Wang Ming,Zhu Lingyun,Liu Jing,Wu Zhiguang,Guo Yupu (College of Veterinary Medicine,Chinese Agricultural University,Beijing 100094,China);CLONING AND SEQUENCING ANALYSIS OF CHINESE HUIYANG CHICKEN IFN α GENE[J];CHINESE JOURNAL OF ANIMAL AND VETERINARY SCIENCES;2000-06
3 GUO Yu-fei,CHENG An-chun,WANG Ming-shu,JIA Ren-yong, WEN Ming,ZHOU Wei-guang,CHEN Xiao-yue (Research Center of Poultry Diseases,Sichuan Agricultural University, Ya'an 625014, China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province,Ya 'an 625014 , China; College of Animal Science,Guizhou University,Guiyang 550025, China;College of Animal Science and Veterinary Medicine, Nei Monggol Agricultural University,Hohhot 010018, China);Establishment and application of quantitative real time PCR assay for detecting duck viral enteritis virus[J];Veterinary Science in China;2006-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved