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《Chinese Journal of Veterinary Medicine》 2014-01
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Determination of Doramectin in Plasma of Sheep by HPLC with Fluorescence Detection

SHI Yan-li;HAN Cai-xia;ZHANG Zi-qun;LI-Xiao-yun;SONG Ming-xin;LI Wei;College of Veterinary Medicine,Northeast Agricultural University;Heilongjiang Entry-Exit Inspection and Quarantine Bureau;  
A novel method was developed to determinate doramectin in plasma of sheep using high-performance liquid chromatography(HPLC) with fluorescence detection.Doramectin in plasma of sheep was extracted using acetonitrile,purified by solid phase extraction on a 0DS-C18 column,and dried in airless box.Subsequently,it was modified with trifluoroacetic anhydride and N methyl glyoxaline at room temperature.After being injected into the HPLC column,the doramectin was detected by fluorescence detector under 365 nm excitation and 475 nm emission wave lengths.The limit of quantitation was 0.1 ng/mL.The method generated good linear calibration graphs within the concentrations from 1 to 100 ng / mL.The mean recovery rates of Doramectin ranged from89.50%to 95.67%.The coefficient of variability within one day and among several days was smaller than 4%and 5%,respectively.This study provides a rapid,reliable,and sensitive method for determination of doramectin in plasma of sheep.
【Fund】: 哈尔滨市科技创新人才研究专项资金(2006RFQXN016);; “十一五”黑龙江省科技攻关计划项目(GA06B202-3)
【CateGory Index】: S858.26
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