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《CHINESE JOURNAL OF VETERINARY MEDICINE》 1994-01
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Studies on Indirect Dot-ELISA for the Detection of Haemophilus paragallinarum(Hpg)Antigens

Wang Leyuan; Gan Menghou  
This study establishes for the first time a standardized procedure using indirect Dot-ELISAfor the detection of Hpg antigens. The amounts detected were 3.91 ×10 ̄5 CUF/ml of Hps. The antigen-posi-tive rates of Hpg samples collected form experimentally infected chickens were oral swab 95.5%,nasal and si-nus exudate swab 90.9%,with no statistically significant difference between them. The antigen-positive ratesof oral swabs collected post infection at 4 days and 8 days and detected by indirect Dot-ELISA were 100%and95.5%,and also had no statistically significant difference. The antigen-positive frequency of Hpg samples col-lected from field infection were oral swab 81.7%,nasal exudate swab 77.8%,sinus exudate swab 76.9%.The indirect effect-ELISA is an economical,quick ,easy-to-perform,sensitive,specific and reproducible test.It’suseful for early diagnosis of IC. and is also an efficient tool for epidemilogical investigations of infectious dis-eases.
【CateGory Index】: S858.315.1
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【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 XIE Chun-fu1,LIU Hong1,LI Qing-suo1,LI Jie-feng1,FENG Ai-shu2(1.Hebei Provincial Husbandry and Veterinary Research Institute,Baoding,Hebei,071000,China;2.Xingtai Municipal Bureau of Agriculture,Xingtai,Hebei,054000,China);Preparation and Immunization Test of Chicken Infectious Coryza Inactivated Vaccine[J];Progress in Veterinary Medicine;2008-08
2 LI Jie-feng1,XIE Chun-fu1,LIU Hong1,LI Qing-suo1,LIANG Zhan-jun2,GAO Wei-fang3(1.Hebei Provincial Husbandry and Veterinary Research Institute,Baoding,Hebei,071000,China;2.The Bureau of Wuji Raising Livestock,Wuji,Hebei,052460,China;3.The Bureau of Wen'an Raising Livestock,Wen'an,Hebei,065800,China);Pathogenicity and Growth Property of Haemophilus paragallinarum Isolate[J];Progress in Veterinary Medicine;2009-04
3 GONG Yu-mei1,ZHANG Pei-jun1,ZHU Wen-ge2,SUN Hui-ling1,WANG Hong-jun1,HE Yun-xia1,SHAO Qing-hong2,XU Hui1(1.Institute of Animal Husbandry and Veterinary Science,Beijing Academy of Agricultural and Forestry Science,Beijing,100097,China;2.Beijing Sinder Vet-Technology CO.,LTD.Shunyi,Beijing,101302,China);Safety and Efficacy Study on Trivalencs Inactivated Vaccines Against Infectious Coryza[J];Progress in Veterinary Medicine;2010-03
4 GONG Yu-mei,ZHANG Pei-jun,SUN Hui-ling,WANG Hong-jun,HE Yun-xia(Institute of Animal Husbandry and Veterinary Science,Beijing Academy of Agricultural and Forestry Science,Beijing 100097,China);Evaluation of Three Strains Serotype of Avibacterium paragallinarum Virulence to SPF Chicken[J];Progress in Veterinary Medicine;2011-02
5 Zhang Huan,Hou Jia-lei,Zhang Yan-hong,Luo Jing-lu,Ren Tao(Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China);Establishment of a PCR Detection Method for Haemophilus Paragallinarum[J];Guangdong Journal of Animal and Veterinary Science;2009-01
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Chinese Journal Full-text Database 10 Hits
1 MIAO De yuan 1,ZHANG Pei jun 1,GONG Yu mei 1,FENG Wen da 2,FENG Shan 2 (1 Institute of Animal Husbandry and Veterinary Science,Beijing Academy of Agricultural and Forestry Sciences ,Beijing 100089,China; 2 The National Control Institute of Ve;Tests of Efficacy of Bivalent Inactivated Oil Adjuvant Vaccine to Avian Infectious Coryza[J];ACTA AGRICULTURAE BOREALL—SINICA;2000-02
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【Secondary References】
Chinese Journal Full-text Database 6 Hits
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2 MIAO De yuan 1 ,ZHANG Pei jun 1,GONG Yu mei 1,MIAO De rong 2 (1.Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100089,China; 2.Luyuan Company,Shandong Lukang Pharmaceutica;Studies on Dynamics of Antibodies to Haemophilus Paragallinarum in Sera from Chickens Vaccinated with Bivalent Inactivated Oil Adjuvant Vaccine to Infectious Coryza[J];Acta Agriculturae Boreall—sinica;2001-03
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4 WANG Hong-jun~(1,2),ZHANG Pei-jun~2,GONG Yu-mei~2,YANG Han-chun~(1*)(1.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China;2.Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture andForestry Sciences,Beijing 100097,China);Cloning and Expression of HA Gene of Serum-type B of Haemophilus paragallinarum in E.coli and Study on Biological Activity of Recombinant Protein[J];Acta Veterinaria et Zootechnica Sinica;2007-01
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