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《Journal of Traditional Chinese Veterinary Medicine》 2018-05
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Determination of ellagic acid in Yunshi Ganmao Mixture made from Guizhou Miao medicine Geum japonicum

WANG Zheng;JIN Yao;WANG Jiazhi;SUN Yuwen;Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Hubei University of Medicine;Department of Emergency,Taihe Hospital,Hubei University of Medicine;  
Objective:To establish a method for determining ellagic acid in Yunshi Ganmao Mixture. Methods:The chromatographic separation was performed on the Phenomenex Synergi column(2.0 mm×100 mm,2.5 μm)with gradient elution of methanol and 0.1% aqueous formic acid at a flow rate of 0.3 mL/min. The column temperature was set at 40 ℃,the detection wavelength was 254 nm,and the injection volume was 3 μL. Results:The method for determining ellagic acid in the same chromatographic condition was set up,which showed good linearity in the test ranges. Conclusion:The methods developed are convenient,rapid,accurate and reproducible,which is applicable to the quality control of Yunshi Ganmao Mixture.
【Fund】: 湖北医药学院校基金(自然科学研究)资助计划项目(2015QDJZR14)
【CateGory Index】: S853.7
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