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《Plant Protection》 2014-01
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Isolation and identification of Bacillus atrophaeus antagonistic against Verticillium dahliae

Sun Chongsi;Chen Xiaomin;Shu Changlong;Qi Fangjun;Gao Jiguo;Zhang Jie;College of Life Sciences,Northeast Agricultural University;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute ofPlant Protection,Chinese Academy of Agricultural Sciences;  
The purpose of this study is to isolate the bacteria antagonistic to Verticillium dahliae,an important plant disease,from soil samples collected in cotton fields in Xinjiang Uygur Autonomous Region,and to identify bacterial species and discuss their application prospect.Antagonistic assay on the plates was conducted for the initial screening using strain culture and fermentation filtrate,and then the stability of heat treatment for fermentation filtrate was detected.16S rDNA and gyrBgene sequence analyses were used to classify antagonistic bacteria. Sixty-one strains inhibiting V.dahliae were obtained.Among them,No.59 strain had highly antagonistic activity to V.dahliae,and could produce pigment.Its fermentation filtrate still kept antagonistic activity after heated at 100 ℃for 5 min,and the diameter of fungistasis circle of the heated sample was 15.8 mm.The strain was antagonistic against V.dahliae in pot tests,and promoted growth of cotton seedlings.The results indicated that No.59 strain belonged to Bacillus atrophaeus through phylogenetic tree analysis based on16S rDNA combined with gyrB gene sequences.This is the first report that B.atrophaeus had antagonistic activity against V.dahliae.
【Fund】: “十二五”国家“863”计划课题(No.2011AA10A203)
【CateGory Index】: S476
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