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《Plant Protection》 2014-01
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Identification of potato wilt caused by Fusarium avenaceum and the biological characteristics of its pathogen

Wang Yuqin;Yang Chengde;Chen Xiurong;Xue Li;Su Jianhong;College of Grassland,Gansu Agricultural University;Key Laboratory of Grassland Ecosystem,Ministry of Education;Pratacultural Engineering Laboratory of Gansu Province;Sino-U.S.Center for Grazingland Ecosystem Sustainability;  
The pathogen causing potato wilt were isolated and identified by the rDNA-ITS sequences and biological characteristics in Gansu Province.The results showed that the macroconidia were hyaline,sickle-shaped,multicellular,and the size was(12.5-30)μm ×(2.5-5)μm.The microconidia were oval,and the size was(4.70-12.94)μm×(1.76-2.35)μm.The rDNA-ITS sequence of the pathogenic fungus shared 100% similarity with that of Fusarium avenaceum(JN6317483).The morphologic characteristics and ITS sequence analysis all indicated that the pathogenic fungus was F.avenaceum.The suitable culture temperature was from5 ℃to 40 ℃,and the optimum was 20℃.D-fructose and leucine had significant promoting effect on the growth of the mycelia(P 0.05),and chloralose and ammonium carbonate significantly inhibited mycelial growth(P0.05).Oatmeal medium played a significant role in promoting mycelial growth(P0.05).
【Fund】: 甘肃省农牧厅项目
【CateGory Index】: S435.32
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