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Genetic diversity of Candidatus Liberibacter isolates from different geographical regions of Guangdong Province

Huang Yonghui;Cheng Baoping;Peng Aitian;Song Xiaobing;Ling Jinfeng;Chen Xia;Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangdong Provincial Key Laboratory of High Technology for Plant Protection;  
Huanglongbing(HLB)is a destructive disease on citrus,and pathogens with different genotypes may possess different pathogenicity.Ascertain the distribution of CandidatusLiberibacter asiaticus with different genotypes in Guangdong Province could provide an important basis for the studies of disease epidemics.In this study, PCR-RFLP analysis based on the outer membrane protein gene(omp)was conducted with disease samples from different regions.These ompsequences were further sequenced and phylogenetic tree was constructed.The amplified omp genes of Ca.Liberibacter asiaticus were digested with four different restrictive endonucleases and several different gene polymorphisms were produced.Each restrictive endonuclease generated two types of DNA bands, and the pathogen isolates from Guangdong Province all belonged to Ca.Liberibacter asiaticus.There existed no obvious distribution feature among HLB pathogens with different genotypes,suggesting that there were no dominant species of HLB pathogen in Guangdong Province.
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