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《Plant Protection》 2014-01
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Ultrastructure observation and electroantennogram response of Conogethes punctiferalis antennae to corn silk volatiles

Li Qian;Zhang Tiantao;Bai Shuxiong;He Kanglai;Wang Qinying;Li Yunlong;Wang Zhenying;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;College of Plant Protection,Agricultural University of Hebei;Plant Protection Station of Beijing;  
In order to understand the antennal sensilla of Conogethes punctiferalis and their morphological characteristics,and test its antennae response to corn silk volatiles.Scanning electron microscope(SEM)was used to observe the sensilla of C.punctiferalis,and electroantennogram(EAG)was used to test the antennae response to 5 kinds of corn silk volatiles.The results indicated that most of the sensilla are located on the ventral and latero-ventral side of antennae.Among clavola,scape and pedicel,only clavola have reticulate structure.Seven types of sensilla were identified in both sexes of C.punctiferalis antennae,including sensilla trichodea(typeⅠ and type Ⅱ),sensilla chaetica,sensilla auricillica,sensilla campaniform,sensilla basiconica,sensilla coeloconica(typeⅠ and typeⅡ)and sensilla styloconica(typeⅠand typeⅡ).In electroantennogram experiment,antennae showed EAG response raised with the increasing of the concentration of the volatiles tested.Female antennae highly responded to 1-heptanol,β-pinene andβ-ionone,especially to 1-hexanol.Similarly,male antennae also responded to 1-hexanol and 1-heptanol.Females showed highly greater response than males.
【Fund】: 现代农业产业技术体系建设专项(CARS-02);; 北京市科技计划项目(Z111100056811009)
【CateGory Index】: S433.4
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