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Cloning and expression profiles of arginine kinase and GSTs of Nephotettix cincticeps

Hou Jixiang;Meng Panpan;Ma Weihua;Zhou Fei;Lin Yongjun;National Key Laboratory of Crop Genetic Improvement in Wuhan,Huazhong Agricultural University;  
The green leafhopper Nephotettix cincticeps is a rice insect that can transmit plant viruses.We cloned and analyzed an arginine kinase gene named NcAKand a delta class GSTs gene named NcGSTin Nephotettix cincticeps.NcAKcontained an open reading frame(ORF)of 1 068 bp in length,and encodes a protein of 356 amino acids.Phylogenetic analysis showed that the deduced protein of NcAK was closely related to the arginine kinase of Homalodisca vitripennis and Oncometopia nigricans.The real-time PCR results indicated that the NcAK was expressed higher in male adults than in the eggs,1st instars,5th instars,and female adults.NcGST,899 bp in length,contained an ORF of 651 bp and encoded a protein of 217 amino acids.Phylogenetic analysis showed that the deduced protein of NcGST was closely related to the GSTs of Lygus lineolaris.The real-time PCR results indicated that NcGST was expressed higher in 5th instars,male adults and female adults than in eggs and 1st instars.
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