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Preliminary identification of Rhizoctonia solani causing mung bean seedling blight

Wang Yan;Cao Zhimin;Zhang Zhixiao;Su Qiuzhu;Fan Baojie;Liu Changyou;Tian Jing;Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences/Laboratory of Crop Genetic and Breeding of Hebei Province;  
Ninety isolates of Rhizoctonia solani,collected from the mung bean field in Shijiazhuang region were characterized by morphology,anastomosis group and pathogenicity test.The results showed that all isolates were multinucleate.Seventy-one isolates belonged to the AG4,accounting for 78.89%of the tested isolates,55isolates were completely fused with AG4(77.46%),16 strains were incompletely fused with AG4(22.54%).Two isolates belonged to the AG2-2,accounting for 2.22% There were 17 strains that could not fuse with any standard strains,accounting for 18.89%.Virulence test in greenhouse demonstrated that the isolates R3,R6,R9 and R35 were highly virulent,but isolates R23 and R31-1 were weakly virulent.There were extremely significant difference between the isolates R3,R6,R9,R35 and the others belonging to AG-4,also between the isolates R20,R29 and R24 belonging to unknown group.The isolates R21 and R31-1 belonging to AG2-2 were weakly virulent and had no significant difference in virulence.
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