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《Plant Protection》 2014-02
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Establishment and application of PCR for detection of Plasmopara viticola

Qin Wentao;Huang Xiaoqing;Kong Fanfang;Wang Zhongyue;Zhang Hao;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;  
This study designed a pair of primers F-Pv/R-Pv for Plasmopara viticola detection based on the differences among sequenced and reported cox2 gene sequences of P.viticola.Totally 30 kinds of plant pathogens,including 15 kinds of fungi belonging to Plasmopara and 8 kinds of grape pathogens,were screened to validate the primers F-Pv/R-Pv.The results showed that the primers F-Pv/R-Pv were of high specificity,and only a single product of about 600 bp was amplified from P.viticola genomic DNA.The sensitivity of the detection was 3.3 pg/μL genomic DNA per 20μL PCR reaction volume.Genomic DNAs of 78 samples from different grape plantation areas in China were specifically detected by PCR assay with F-Pv/R-Pv,suggesting that this method was applicable to a wide scope with100% detection accuracy rate.
【Fund】: 公益性行业(农业)科研专项(201203035);; 国家现代农业产业技术体系专项基金(nycytx-30)
【CateGory Index】: S436.631
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