Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Plant Protection》 2014-02
Add to Favorite Get Latest Update

Optimization and establishment of SRAP-PCR reaction system for the brown planthopper

Zhang Lei;Jing Shengli;He Guangcun;Jiang Lei;Li Gang;Liu Hong;Qin Rui;Engineering Research Center for Protection and Utilization of Biological Resources in Minority Regions,South-Central University for Nationalities;State Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University;  
By the orthogonal experimental design L16(45),five factors,including Mg2+,dNTPs,Taq DNA polymerase,primer concentration and DNA template concentrations in Nilaparvata lugens SRAP-PCR reaction system,were optimized,and the optimal reaction system of the brown planthopper SRAP-PCR was established.The results showed that a suitable SRAP-PCR reaction system was constructed with10μL volume containing 25 ng of template DNA,0.3μmol/L of each of the two primers,150μmol/L deoxynucleotide triphosphates(dNTPs), 3 mmol/L MgCl2,1× PCR buffer,and 2 unit of Taq DNA polymerase.We used the females of biotypes 1 and the males of biotype 2 monomer hybridization population for the optimal reaction system.The results showed that the bands were clear,with rich polymorphism and co-dominant bands.It showed that the reaction system is stable and reliable.
【Fund】: 湖北省自然科学基金重点项目(2011CDA077)
【CateGory Index】: S435.112.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 SUN Zu-xia1,LIU Zhao-lei1,CHEN Su-mei1,CHEN Fa-di1,LOU Wang-huai1,GUO Hai-lin1,2(1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Botany, Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Optimization and establishment of SRAP-PCR reaction system for Nelumbo nucifera Gaertn[J];南京农业大学学报;2011-06
2 SHAO Qing-song~ 1,2 GUO Qiao-sheng~1 FANG Hai-ling~1(1.Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095;2.Zhejiang Forestry College,Hangzhou 311300);OPTIMIZATION OF SRAP-PCR REACTION SYSTEM FOR Chrysanthemum morifolium Ramat BASED ON THE ANALYSIS OF VARIANCE[J];核农学报;2009-05
3 YANG Ying-hua1,2,LI Xian-xin3,4,ZENG Bo-qian1,5,DENG Zi-niu1,2(1.College of Horticulture and Landscape,HNAU,Changsha 410128,PRC;2.Changsha Branch of State Orange Improving Center,Changsha 410128,PRC;3.Longping Branch of Central South University,Changsha 410125,PRC;4.Hunan Horticultural Reseach Institute,Changsha 410125,PRC;5.Institute of Life Science and Technology,Central South University of Forestry & Technology,Changsha 410004,PRC);The Principle and Research Advancement of Sequence-related Amplified Polymorphism[J];湖南农业科学;2009-05
4 QUAN Jun-ping1,2,YUAN Juhong2,3,MU Hong-mei2,ZHANG Ming-xia2,LI Nai-wei2,XIA Bing2,①(1.College of Agriculture,Shihezi University,Shihezi 832000,China;2.Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China;3.Resource and Environment Management Institute, Jiangxi University of Finance and Economics,Nanchang 330032,China),;Establishment and optimization of ISSR-PCR and SRAPPCR reaction systems of Thymus L.[J];植物资源与环境学报;2008-02
5 GUAN Hua-Nan,CHI De-Fu ,YU Jia,DONG Jing (Department of life science,Northeast Forestry University,Harbin 150040,China).;Optimization of SRAP-PCR reaction system for Harmonia axyridis using orthogonal design[J];昆虫知识;2008-01
6 YUAN Ju-hong1,2, QUAN Jun-ping1, HU Mian-hao3, SUN Shi1, PENG Feng1, XIA Bing1,① (1. Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing 210014, China; 2. Resource and Environment Management Institute, Jiangxi University of Finance and Economics, Nanchang 330032, China; 3. College of Agriculture and Biology,Shanghai Jiaotong University, Shanghai 201101, China),;Establishment and optimization of SRAP-PCR amplification system for Lycoris radiata[J];植物资源与环境学报;2007-04
7 GUO Da-long, LUO Zheng-rong*(Key Laboratory For Horticultural Plant Biology, MOE, Huazhong Agricultural University, Wuhan, Hubei 430070 China);Optimization of SRAP -PCR in some Diospyros spp.[J];果树学报;2006-01
8 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];分子植物育种;2004-05
9 GUAN Xiu_Jie 1,FU Qiang 1*,WANG Gui_Rong 2,LAI Feng_Xiang 1, ZHANG Zhi_Tao 1*(1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094);The DNA polymorphism of host-associated populations of Nilaparvata lugens (Stl) with different virulence[J];昆虫学报;2004-02
10 XU Xiao feng 1,CHENG Xia nian 1,ZU Yun ding 2 (1 Nanjing Agricultural University, 210095;2 Anhui Agricultural University);Analysis on RAPD of Genome DNA in Different BPH Biotypes[J];安徽农业大学学报;2000-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU Wenqiang;WANG Xiaoqian;ZAI Linan;XU Yun;HUANG Xiaolong;HUANG Dongyi;College of Agriculture, Hainan University;;Research Progress of Genetic Diversity on Dioscorea L.[J];热带农业科学;2015-10
2 Wang Guoli;Qiu Yuxiong;Fan Hongying;Department of Life Science, Huizhou University;;Establishment and Application of PCR Detection System of Genetically Modified Maize in Laboratory[J];基因组学与应用生物学;2015-08
3 HAN Zhencheng;PAN Xuejun;AN Huaming;ZHANG Wen'e;Guizhou Engineering Research Center for Fruit Crops,Guizhou University;;Genetic diversity of Diospyros Linn. in Guizhou based on SRAP[J];果树学报;2015-05
4 Mou Dan;Tang Daocheng;Tang Nan;Plateau Flower Research Center of Qinghai University;;Establishment and Optimization of SRAP-PCR System for Male Sterile Lines of Tagetes erecta L.[J];分子植物育种;2015-06
5 SONG Jia;ZHANG Shao-Peng;SUN Qiao;LI Tian-Pei;ZENG Wan-Yong;CHEN Ping;School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University;;Optimization by SRAP-PCR reaction system in rare medicinal herb Panax japonicus C. A. Mey[J];武汉轻工大学学报;2015-02
6 WU Zheng-Qian;LI Xue-Hu;LIANG Jian-Ping;LU Xi-Hong;XIN Zhi-Jun;ZHOU Xiang;Institute of Modern Physics,Chinese Academy of Sciences;College of Veterinary Medicine,Gansu Agricultural University;;Optimizing Sequence-Related Amplified Polymorphism Amplification System for Isatis indigotica Fort[J];植物研究;2015-02
7 He Renfeng;Feng Shangguo;Chen Zhe;Shen Xiaoxia;Shen Yufeng;Wang Zhian;Wang Huizhong;College of Life and Environmental Sciences,Hangzhou Normal University,Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants;Zhejiang Institute of Chinese Meteria Medica;;Optimization and Primer Screening of SSR-PCR Reaction System in Medicinal Chrysanthemum morifolium Based on Orthogonal Design[J];分子植物育种;2015-02
8 CHEN Mei-xia;ZHU Wen-ning;SUN Huan;WANG Jun-hong;WEI Ri-feng;LIU Wei;Ningde Normal University;Henan Kedi Foodstuffs Group Co., Ltd.;Fujian Agriculture and Forestry University;;Establishment and optimization of SRAP reaction system for Dendrobium[J];宁德师范学院学报(自然科学版);2015-01
9 WANG Huijun;WANG Wenquan;LU Cheng;WANG Haiyan;CHEN Xin;Qiongzhou University;Chinese Academy of Tropical Agricultural Sciences/Institute of Tropical Bioscience and Biotechnology;;Optimization of SRAP System and Selection of PCR Primer Combinations in Hevea brasiliensis[J];贵州农业科学;2015-02
10 LAI Maoliang;SUN Renyi;CHENG Jiangbo;NIE Fengqin;XIN Daiwang;LIN Daozhe;FU Wenying;College of Agronomy,Hainan University;;DNA Extraction and SRAP Clustering Analysis of Morinda citrifolia[J];热带生物学报;2014-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Feng1,2,LI Da1,LV Chang-pin2,ZHANG Li1, FENG Yue-dong1,XU Wei1,ZHANG Hong-zhi1(1.College of Horticulture and Landscapes,HNAU,Changsha 410128,PRC; 2.Office of Academic administration,HNAU,Changsha 410128,PRC);The Establishment of Cymbidium DNA Fingerprint Based on SRAP Analysis[J];湖南农业科学;2011-03
2 ZHANG Xian-yan,CHEN Fa-di,ZHANG Fei,CHEN Su-mei,FANG Wei-min(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China);Analysis of genetic diversity among different geographical populations of wild species of Dendranthema[J];南京农业大学学报;2011-01
3 GUO Cai-jie1,2,HOU Li-xia1,CUI Na2,HAN Ming-li2(1Institute of Vegetables,Shandong Academy of Agricultural Sciences,Key Lab for Biology of Greenhouse Vegetable of Shandong Province,National Center for Vegetable Improvement(Shandong),Jinan 250100,Shandong,China;2Biological Science and Technology College,Shenyang Agricultural University,Shenyang 110161,Liaoning,China);Optimization of SRAP-PCR System for Tomato Based on Orthogonal Design[J];中国蔬菜;2011-02
4 SHAO Qing-song~ 1,2 GUO Qiao-sheng~1 FANG Hai-ling~1(1.Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095;2.Zhejiang Forestry College,Hangzhou 311300);OPTIMIZATION OF SRAP-PCR REACTION SYSTEM FOR Chrysanthemum morifolium Ramat BASED ON THE ANALYSIS OF VARIANCE[J];核农学报;2009-05
5 ZHANG Fei,CHEN Fa-di①,FANG Wei-min,LI Feng-tong,LIU Pu-sheng(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China);Optimization and establishment of SRAP-PCR reaction system of Dendranthema×grandiflorum[J];植物资源与环境学报;2009-03
6 LI Mei1,2,HOU Xi-lin1,①,HAO Ri-ming3(1.State Key Laboratory of Crop and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China;3.Horticultural College,Nanjing Agri-cultural University,Nanjing 210095,China);Establishment and verification of SRAP-PCR amplification system for Osmanthus fragrans[J];植物资源与环境学报;2009-02
7 LI Ting-chun~1 FAN Hong-hong~2 GAO Zheng-liang~1 ZHOU Ying-bing YANG Hua-ying(1.Tobacco Research Institute of Anhui Provincial Agricultural Science Academy, Hefei,Anhui 230031;2.School of Life Science,Anhui Agriculture University,Hefei,Anhui 233211);STUDIES ON GENETIC DIVERSITY OF Salvia miltiorrhiza BUNGE WITH A MOLECULAR MARKER SRAP[J];核农学报;2008-05
8 QUAN Jun-ping1,2,YUAN Juhong2,3,MU Hong-mei2,ZHANG Ming-xia2,LI Nai-wei2,XIA Bing2,①(1.College of Agriculture,Shihezi University,Shihezi 832000,China;2.Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China;3.Resource and Environment Management Institute, Jiangxi University of Finance and Economics,Nanchang 330032,China),;Establishment and optimization of ISSR-PCR and SRAPPCR reaction systems of Thymus L.[J];植物资源与环境学报;2008-02
9 LI Long-yun,CHEN Da-xia,QIN Song-yun,LI Quan-sen,ZHONG Guo-yue(Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China);Studies on genetic relationship of seven species of Curculigo plant from China using SRAP[J];中国中药杂志;2008-02
10 FAN Hong-hong1,LI Ting-chun2,QIU Jing1,LI Zheng-peng1,3,LIN Yi1,CAI Yong-ping1(1.School of Life Science,Anhui Agriculture University,Heifei 230036,China;2.Anhui Provincial Tobacco Research Institute,Fengyang 233100,China;3.School of Life Science,Anhui Science and Technology University,Fengyang 233100,China);Studies on genetic diversity of medicinal Dendrobium by SRAP[J];中国中药杂志;2008-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved