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《Plant Protection》 2014-03
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Screening of herbicidal fungi from the gut of Oxya chinensis and identification of the strain DH03

Lan Tian;Chen Tingting;Shao Mingwei;Fan Qiuqin;Pan Qianqian;Wen Dan;Zhang Yinglao;College of Chemistry and Life Sciences,Zhejiang Normal University;  
Six fungi were isolated from the gut of Oxya chinensis by plate separation method.The phytotoxic experiments suggested that the strain DH03 had the best herbicidal activity.When the mass concentration was 100μg/ mL,the inhibition rates of EtOAc extract against the roots of Echinochloa crusgalli and Amaranthus retroflexus were more than 80%,comparable to that of 2,4-dichlorophenoxyacetic acid.Further research showed that the main herbicidal ingredients of fermentation broth were in the moderate polar part.Moreover,its fermentation broth showed a strong inhibitory effect on growth of E.crusgalli.Sprayed with fermentation broth of DH03, victimization rate and average plant height were 36.1% and 9.78 cm,respectively.The strain was identified as Cladosporium oxysporum by the morphological features and ITS sequence analysis.As a microbial herbicide,it is worthy of further study.
【Fund】: 国家自然科学基金(21272215 21002092);; 浙江师范大学校级课题(KJ20120026)
【CateGory Index】: S476.1
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