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《Plant Protection》 2014-03
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Effective universal vectors building for gene knockout and fluorescent expression in Fusarium graminearum

Wang Xiaoliang;Zhang Hao;Pan Yi;Xu Jingsheng;Xu Jin;Feng Jie;State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;  
A series of effective universal vectors for gene knockout and fluorescent expression in Fusarium graminearum were constructed by a USER enzyme based technique.The gene knockout vector pKH-KO were constructed by insertion of two USER cloning sites(UCS)into the two sites of hph gene and the fluorescent expression vectors pKHGFPE and pKNGFPE were constructed by insertion of UCS into the 5′side of gfp gene.This method has many advantages,such as a simple experimental design,few procedures,user convenience and less time-consuming,which has a broad application prospect in gene function research such as large scale gene-knockout and fluorescent protein localization.
【Fund】: 国家自然科学基金项目(31201477);; 国家国际合作专项(2013DFG31930);; 公益性行业(农业)科研专项(201303016)
【CateGory Index】: Q782
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