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《Plant Protection》 2014-03
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Effective universal vectors building for gene knockout and fluorescent expression in Fusarium graminearum

Wang Xiaoliang;Zhang Hao;Pan Yi;Xu Jingsheng;Xu Jin;Feng Jie;State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;  
A series of effective universal vectors for gene knockout and fluorescent expression in Fusarium graminearum were constructed by a USER enzyme based technique.The gene knockout vector pKH-KO were constructed by insertion of two USER cloning sites(UCS)into the two sites of hph gene and the fluorescent expression vectors pKHGFPE and pKNGFPE were constructed by insertion of UCS into the 5′side of gfp gene.This method has many advantages,such as a simple experimental design,few procedures,user convenience and less time-consuming,which has a broad application prospect in gene function research such as large scale gene-knockout and fluorescent protein localization.
【Fund】: 国家自然科学基金项目(31201477);; 国家国际合作专项(2013DFG31930);; 公益性行业(农业)科研专项(201303016)
【CateGory Index】: Q782
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【Citations】
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【Co-citations】
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1 Wang Fenghuan1 Meng Qingqing1 Feng Di1 Yang Jianguo2 Wang Bing3(1Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients,Beijing Key Laboratory of Food Flavor Chemistry,School of Food and Chemical Engineering,Beijing Technology and Business University(BTBU),Beijing 100048;2College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029;3Beijing Zhongtian Nuoya Enzyme Co.,Ltd.,Beijing 102629);Expression of Thermostable Glucoamylase Gene in Aspergillus niger and Its Characterization[J];Biotechnology Bulletin;2012-07
2 LIU Xiao-lin,JIANG Xian-zhang,YU Qing-mei,ZHANG Ming-liang,HUANG Jian-zhong(Enginring Research Center of Fujian Modern Ferment Technology, College of Life Science,Fujian Normal University,Fuzhou 350108,China);Bioinformatics analysis of Delta-12 desaturase in filamentous fungus[J];Journal of Yunnan University of Nationalities(Natural Sciences Edition);2013-01
3 Zhang Lilin 1,Cao Huijuan 2,Li Xiaodong 1,Lin Fucheng 2,3,Feng Xiaoxiao 2,Lu Jianping 1 (1 School of Life Science,Zhejiang University,Hangzhou 310058,China;2 Biotechnology Institute,Zhejiang University,Hangzhou 310058,China;3 China Tobacco Gene Research Center,Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China);MoTCTP, A Homolog of Translationally Controlled Tumor Protein, Is Required for Fungal Growth and Conidiation in Magnaporthe oryzae[J];Chinese Journal of Cell Biology;2013-08
4 LI Xu-you;Hubei Vocational College of Ecological Engineering;;Application of modern biotechnology in antibiotic production[J];Journal of Wuhan Institute of Technology;2014-01
【Secondary Citations】
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1 LIU Li\+1\ LIU Jin\+2\ QIU Run-Xiang\+1\ ZHU Xing-Guo\+1\ TANG Guo-Min\+ 1* 1(Institute of Microbiology, china Academy of Sciences, Beijing 100080, China) 2(Shandong University, Jinan 250001,China);Construction of Recipient Strain of Expression-secretion System in Filamentous Fungi[J];Chinese Journal of Biotechnology;2002-06
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8 WANG Jiao-Yu1, 2, DU Xin-Fa1, CHAI Rong-Yao1, SUN Guo-Chang1, LIN Fu-Cheng2 1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China; 2. Colledage of Agriculture and Biotechnology, Zhejiang Universtiy, Hangzhou 310029, China;Strategies of targeted gene replacement in filamentous fungi[J];Hereditas;2007-07
9 WANG Hong kai 1 , LIN Fu cheng 2 , CHAI Rong yao 3 , LI De bao 1 ( 1 Biotechnology Institute, Zhejiang University, Hangzhou 310029, China; 2 College of Life Sciences,Zhejiang University, Hangzhou 310029, China; 3 Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China);Screening of Temperature Sensitive Mutants in Magnaporthe grisea[J];Chinese Journal of Rice Science;2004-04
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