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《Chinese Journal of Gastrointestinal Surgery》 2002-04
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Raising of polyclonal antibody against motilin receptor and its expression in human stomach

WANG Zhigang, QIN Xinyu, SHI Jiahao, et al. Department of General Surgery, Zhongshan Hospital, Fudan University,Shanghai 200032,China  
Objective To raise polyclonal antibody against motilin receptor and investigate the expression of this receptor in human stomach. Methods Synthesized peptide was used to raise rabbit anti human antibody against N terminal of motilin receptor and ELISA method was used to measure the titer of the antiserum. Electro transfect 293 cells were transfected with GPR38 plasmid and RT PCR was used to confirm successful transfection. Western blot and immunohistochemistry method were applied to study protein expression of motilin receptor in transfected 293 cells and human stomach tissues. Results The titer of raised antibody was 1∶500 000 with good specificity. GPR38 plasmid was successfully transfected in 293 cells with protein expression. Motilin receptor was shown to express in glandular epithelium of antrum and corpus of stomach. Conclusions Polyclonal antibody against motilin receptor is successfully raised by synthesized peptide with high titer and good specificity. Motilin receptor expresses in glandular epithelial cells of antrum and corpus of human stomach.
【Fund】: 国家自然科学基金资助项目(30070738)
【CateGory Index】: R392.11
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【References】
Chinese Journal Full-text Database 1 Hits
1 PAN Lili;GAO Shanling;YIN Jibin;ZHANG Wenhua;Department of Gastroenterology,the Second Affiliated Hospital of Harbin Medical University;;The distribution of motilin receptor and the relationship with diseases[J];胃肠病学和肝病学杂志;2013-11
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 WANG Yan-yu,LIU Jun-feng,GONG Hai-feng,GAO Qing-min,CHEN Yong,LI Yun-feng, WNAG Fu-shun,LI Bao-qing.Department of Thoracic Surgery,Fourth Hospital,Hebei Medical University, Shijiazhuang 050011,China;The expression of motilin receptor in lower esophageal sphincter and its contraction responses to motilin[J];中华实验外科杂志;2011-04
2 XU Wen-da,JIANG Xun,LAN Li,TONG Hai-xia,WANG Chun-hui,WANG Bao-xi.Department of Pediatrics,Tangdu Hospital,The Fourth Military Medical University,Xi'an 710038,China;Motilin receptor expression in the interstitial cells of Cajal[J];中华儿科杂志;2011-04
3 YANG Xia, DONG Lei~△, YANG Hao. Department of Gastroenterology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China;Evidence for the Presence of Motilin Receptor and a Study on the Mechanism of Motilin Induced Ca~ 2+ Signaling in Rat Myenteric Neurons[J];四川大学学报(医学版);2006-05
4 LIU Mei1, DONG Lei1, ZHU Wen-yi2 1Department of Gastroenterology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China; 2Department of Orthopedics, Beijing Red-Cross Emergency Center, Beijing 100085, China;Distribution and role of motilin receptor in the amygdala of rats[J];第一军医大学学报;2005-09
5 WANG Zhigang, QIN Xinyu, SHI Jiahao, et al. Department of General Surgery, Zhongshan Hospital, Fudan University,Shanghai 200032,China;Raising of polyclonal antibody against motilin receptor and its expression in human stomach[J];中华胃肠外科杂志;2002-04
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 XU Haigen;ZHONG Lanlan;LI Xiaoxiao;LI Junjie;YANG Xin;GU Jie;JIANG Zhenfei;ZHANG Ying;QIN Xia;School of Anesthesiology,Xuzhou Medical University;Department of Anesthetic Physiology,Xuzhou Medical University;;Effects of different anesthetics on the gastrointestinal motility in alcoholic mice[J];徐州医学院学报;2017-02
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