Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《》 1990-01
Add to Favorite Get Latest Update

The Influence of Proline on the Expansion Potential of Isolated Rye Protoplasts

LIU You-Liang(Nanjing Agricultural University, Nanjing 210014)Steponkus PL(Cornell University, Ithaca, NY 14853 U.S.A.)  
The tolerable surface area increment (TSAI_(50)) of protoplasts isolated from nonacclimated leaves of Secale cereale cv. Puma and suspended in sorbitol or NaCl+CaCl_2 solutions was about 1000 μm~2, and was independent of the extent of contraction of the protoplasts. However, when the protoplasts were suspended in proline solution, the TSAI_(50) changed with the contraction. When the protoplasts from isotonic proline solutions expanded in hypotonic solutions, the TSAI_(50) was approximately 1000 μm~2. However, the protoplasts contracted in 0.83, 1.20 Osm or 2.00sm proline solutions when were placed in bypotonic solutions, the TSAI_(50) increased to 1710±85, 1873±91μm~2 and 2200±283μm~2, respectively. These values approached to these of cold acclimated protoplasts either suspended in hypertonic sorbitol NaCl+CaCl_2 or proline (Table 1). The beneficial effects of proline on non-acclimated protoplasts occurred only during contraction, and had no relation to the solution compositien during expansion (Table 2). The mechanism of cryoprotection by proline was discussed.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved