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Isolation and Expressional Analysis of cDNA Encoding a dsRNA Binding Protein Homologue OsRBP of Rice

TANG Xiang-Rong 1 WU Hao 1 JIA Ming 1 YU Xu-Hong 1 HE Yu-Ke 1 ( 1 Shanghai Institute of Plant Physiology and Ecology,Shanghai Institute for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032; 2 Pudong Agrotechnology Developmental  
Two EST fragments in GenBank were found to be homologous to double-stranded RNA binding domains of BcpLH gene of Chinese cabbage.The primers were designed according to the position of homologous regiogs and RT-PCR was performed using cDNA as the template of callus tissue of rice (Oryza sativa subsp.japonica ZH11).A 1.8-kb diagnostic DNA fragment spanning the complete coding sequence(Fig.1)of OsRBP was generated.It contains two typical dsRBD domains sharing 75% identity in amino acid sequence(Fig.3) and therefore was designated as OsRBP.It is showed by expression analysis of RT-PCR that the expression of was seen in immatued seeds and callus derived from immatured seeds and not found in roots,stems,leaves,spikes and matured seeds(Fig.4).The first repost on dsRNA binding protein gene and its expression pattern makes it possible to study regulatory function of dsRNA binding protein to development of rice major organs.
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