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《Plant Physiology Communications》 2007-01
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Gene Cloning and Sequence Analysis of Thionins from Polygonum sibiricum Laxm.

LIU Chang-Cai1,2, LIU Guan-Jun1,*, LIU Gui-Feng1, LI Xiao-Ze1 1College of Forestry, Northeast Forestry University, Harbin 150040, China; 2 The No. 61699 Army of Chinese People's Liberation Army, ZhiJiang, Hubei 443200, China  
A partial sequence of a thionins (THI) gene was obtained from a random clone in Polygonum sibiricum SSH library and THI was cloned by using rapid amplification of cDNA end (RACE) technology. The acquired gene was 789 bp in full length, including 5' untranslated region of 90 bp, 3' untranslated region of 276 bp with Poly A, and an open reading frame (ORF) encoding 140 amino acid. Sequence analysis indicated that the protein, such as most of the plant THI, was a precursor which could be divided into three distinct domains, including an amino-terminal (N-terminal) signal peptide, a mature THI and a carboxy-terminal (C-terminal) acidic domain, which were composed of 24, 46 and 70 amino acid respectively. THI was found to be highly homologous with that from other dicotyledon. All of them possessed a conserved plant THI signature sequence of "C-C-X(5)-R-X(2)-[FY]-X(2)-C" . The mature THI had positive charge and showed slightly high basic. As result, it was possibly involved in antimicrobial activity and was identified as a new plant THI. The gene accession nucleotide sequence number in GenBank was DQ981482.
【Fund】: 黑龙江省重点攻关项目(GB06B303)。
【CateGory Index】: Q943.2
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