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《Plant Physiology Communications》 2007-01
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Gene Cloning and Sequence Analysis of Thionins from Polygonum sibiricum Laxm.

LIU Chang-Cai1,2, LIU Guan-Jun1,*, LIU Gui-Feng1, LI Xiao-Ze1 1College of Forestry, Northeast Forestry University, Harbin 150040, China; 2 The No. 61699 Army of Chinese People's Liberation Army, ZhiJiang, Hubei 443200, China  
A partial sequence of a thionins (THI) gene was obtained from a random clone in Polygonum sibiricum SSH library and THI was cloned by using rapid amplification of cDNA end (RACE) technology. The acquired gene was 789 bp in full length, including 5' untranslated region of 90 bp, 3' untranslated region of 276 bp with Poly A, and an open reading frame (ORF) encoding 140 amino acid. Sequence analysis indicated that the protein, such as most of the plant THI, was a precursor which could be divided into three distinct domains, including an amino-terminal (N-terminal) signal peptide, a mature THI and a carboxy-terminal (C-terminal) acidic domain, which were composed of 24, 46 and 70 amino acid respectively. THI was found to be highly homologous with that from other dicotyledon. All of them possessed a conserved plant THI signature sequence of "C-C-X(5)-R-X(2)-[FY]-X(2)-C" . The mature THI had positive charge and showed slightly high basic. As result, it was possibly involved in antimicrobial activity and was identified as a new plant THI. The gene accession nucleotide sequence number in GenBank was DQ981482.
【Fund】: 黑龙江省重点攻关项目(GB06B303)。
【CateGory Index】: Q943.2
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Lu Jingmei;Li Jiandong(National Laboratory of Grassland Ecological Engineering,Northeast Normal University,Changchun,130024);SEM Observation of the Anatomical Structure of Polygonum sibricum Laxm[J];JOURNAL OF NORTHEAST NORMAL UNIVERSITY (NATURAL SCIENCE EDITION);1994-03
2 LV Yan-fang1, WANG Da-hai2, LIU Guan-jun3, YANG Chuan-ping3, ZHOU Ying1( 1.College of Biotech and Food Science, Bohai University, Jinzhou 121000, China; 2.Department of Forest Research, China Academy of Forest, Beijing 100091, China; 3.Department of Forest Tree Breed, Northeast Forestry University, Harbin 150040, China );The location patterns of inorganic ions in different parts of Polygonum sibiricum under salt and alkaline stress condition[J];Guihaia;2006-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XIONG Xian-qin1,WANG Ming-jin2,WANG Hai3,WEN Ke-jian1,MENG Jun-jiang1,ZHOU Yu-feng1(1.Guizhou Institute of Prataculture,Guizhou Guiyang,558200,China;2.Bijie Prefecture of Guizhou Province fodder Workstation Bijie,GuiZhou Bijie 551700,China;3.Bijie Grazing Field,Guizhou Dafang 551600,China);Prevent and control technologies for Polygonim sibircum[J];Pratacultural Science;2010-06
2 MA Hong1,2,3,HAN Yujun3,WANG Qianyu4,HU Fan4,TAO Bo3(1.Post-doctoral Research Center,Heilongjiang Academy of Argricultural Sciences,Harbin 150086,China;2.Post-doctoral Research Station,Northeast Forestry University,Harbin 150086,China;3.College of Agriculture,Northeast Agricultural University,Harbin 150030,China;4.Jiamusi Sub-academy,Heilongjiang Academy of Agricultural Sciences,Jiamusi Heilongjiang 154007,China);Study on foliar surface features and dissection constructure of dayflower(Commelina commumis L.)[J];Journal of Northeast Agricultural University;2011-10
3 KANG Lijuan~(1,2),PAN Xiaojie~(1,2),CHANG Fengyi~(1,2),LI Durthai~1,SHEN Yinwu~1 & LIU Yongding~1 (1:Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,P.R.China) (2:Graduate School of Chinese Academy of Sciences,Beijing 100049,P.R.China);Effects of alkalinity variations on photosynthetic activity and exopotysaccharides of Chlorella pyrenoidosa[J];Journal of Lake Sciences;2008-02
4 XIONG Xian-qin1,WANG Ming-jin2*,WANG Hai3,MENG Jun-jiang1,ZHAO Ming-kun1(1.Guizhou Pratacultural Institute,Guiyang,Guizhou 550006;2.Forage and Fodder Station of Bijie Prefecture,Bijie,Guizhou 551700;3.Pasture Farm of Bijie Prefecture,Dafang,Guizhou 551600,China);Ecological Distribution and Characteristics of Polygonim sibircum in Guizhou[J];Guizhou Agricultural Sciences;2010-04
5 WU Kun-xin,ZHANG Xiu-chun,CHEN Xiong-ting(Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou 571101,China);Preliminary study on screening the antifungal proteins from seeds of tropical forages[J];Guangdong Agricultural Sciences;2008-11
6 LIU Yan1,FENG Juan1,TAO Dong-liang3,WENG Shi-fu3,REN Zheng-long1,2 1.School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054,China2.Sichuan Province Key Laboratory of Plant Genetics and Breeding,Sichuan Agricultural University,Yaan 625014,China3.College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China;Study on the Effect of C-Terminal Acidic Protein on the Prokaryotic Expression of α-Thionin by FTIR Microspectroscopy[J];Spectroscopy and Spectral Analysis;2009-12
7 LV Yan-fang1, WANG Da-hai2, LIU Guan-jun3, YANG Chuan-ping3, ZHOU Ying1( 1.College of Biotech and Food Science, Bohai University, Jinzhou 121000, China; 2.Department of Forest Research, China Academy of Forest, Beijing 100091, China; 3.Department of Forest Tree Breed, Northeast Forestry University, Harbin 150040, China );The location patterns of inorganic ions in different parts of Polygonum sibiricum under salt and alkaline stress condition[J];Guihaia;2006-03
8 YANG Mei-juan1,2,YANG De-kui2,LI Fa-zeng2 (1.College of Life Science,Linyi Normal University,Shandong Linyi 276005,China;2.Key Laboratory of Plant Stress,Shandong Normal University,Shandong Jinan 250014,China);Effects of NaCl on the Root of Atriplex centralasiatica Iljin[J];Journal of Hebei Normal University(Natural Science Edition);2007-06
9 Zheng Lei Liu Guanjun Yang Chuanping(Northeast Forestry University) Wang Dahai(the Chinese Academy of Forestry);2-DE ANALYSIS AND MASS SPECTROMETRY IDENTIFICATION OF PROTEINS IN POLYGUMN SIBIRICUM UNDER SALT STRESS[J];Natural Science Journal of Harbin Normal University;2007-02
10 ZHENG Lei LIU Guan-Jun YANG Chuan-Ping*(Key Laboratory of Forest Genetics and Tree Breeding of Heilongjiang Province,Northeast Forestry University,Harbin 150040);Cloning and Sequence Analysis of rd22 Gene from Polygonum sibricum[J];Bulletin of Botanical Research;2007-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Ling-li 1, TAN Yan 1 , LIU Li-hua 1, ZHANG Kun 2, SHI Yang 1, XU Shu-fen 1, FANG Yan-qiu 1, DUAN Xiu-mei 1, JIANG Yan-fang 1, WANG Xiao-qi 1 (1. Department of Central Laboratory, First Hospital, Jilin University, Changchun 130021,China;2. Center of Disease Control and Prevention of Jilin Province, Changchun 130061,China);Splicing overlap extension by PCR:a convenient method to obtain recombinant gene[J];Journal of Jilin University of (Medicine Edition);2004-05
2 SHENG Yan-min,YANG Yan-ping,WU Ying-jie,WANG Qian-qian (School of Life Science,Changchun Normal University,Changchun 130032,China);DNA Polymerase Applied in PCR Technique[J];Journal of Changchun Normal University(Natural Sciences);2008-12
3 Shu Wenhua; Geng Huazhu (Animal science institute,Chinese Academy of Agricultural Science, Beijing, 100094,China) Sun Yongru (Genetic institute, Academia sinica, Beijing, 100101,China);PROTOPLAST CULTURE AND PLANT REGENERATION FROM MESOPHYLL OF ALFALFA (MEDICAGO SATIVA L.)[J];Acta Agrestia Sinica;1994-01
4 Chen Yan; Li Cong; Su Jiakai; Huang Wenhui (Animal Science Institute of CAAS, Beijing 100094) Lu Deyang; Zhao Guangyu (Genetic Institute of Academia Sinica, Beijing 100012);Agrobacterium rhizogenes Mediated Gene Transfer of the Forage Legume Lotus Corniculatus L[J];Acta Agrestia Sinica;1996-02
5 RONG Yu-ping, WANG Kun (Animal Science and Technology College, China Agricultural University, Key and Open Lab of Grassland Restoration and Reestablishment of China Agriculture Ministry, Beijing 10094,China);The review of alfalfa and the bloat in grazing animal.[J];Pratacultural Science;2004-12
6 ZHANG Zhenxia1, CHU Chengcai2, FU Yikun1(1.Gansu Agricultural University, Lanzhou 730070, China; 2. Institute of Genetics and Developmental Biology, Chinese Academyof Sciences, Beijing 100101, China);<title>Transformation of Leguminous forage Lotus corniculatus by Agrobacterium mediated GA 20oxidase gene[J];Acta Pratacultural Science;2002-03
7 LIU Jian-li~(1,2,3),ZHANG Zhan-lu~(1,4),WU Yan-min~1,TANG Yi-xiong~1,GUO Ai-guang~2,CHEN Liang-gui~5(1.Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2.College of Life Sciences,Northwest A&F University,Yangling 712100,China;3.Department of Life Sciences,The second Northwest University for Minorities,Yinchuan 750021,China;4.College of Food Science and Nutritional Engineering,China Agriculture University,Beijing 100083,China;5.Shenzhen Nongke Group,Shenzhen 518040,China);Establishment of an efficient Agrobacterium-mediated genetic transformation system of Lotus corniculatus[J];Acta Prataculturae Sinica;2006-03
8 ZHANG Jing1,ZHOU Chang-mei2( 1. Biotech Department, Liaoning Agricultural Vocation-technical College, Yingkou Liaoning 115214, PRC;2. College of Agriculture, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC );Major approaches for obtain marker-free transgenic plants[J];Journal of Northeast Agricultural University;2004-04
9 Zhang Li Wang Dongfeng Zhang Bin Sun Liping(College of Food Science and Engineering,Ocean University of China,Qingdao 266003);ADVANCES IN THE STUDY OF PROTEASE INHIBITORS FROM LEGUMIN OUS PLANTS[J];Soybean Science;2006-03
10 Wang Hui (Xinjiang Institute of Biologg,Pedology and Desert Research,Chinese Academy of Sciences,Urumqi, 830011 );THE RELATIONSHIP BETWEEN THE BROWNMED AND DISTRIBUTION OF COMPARTMENTATION OF POLYPHENOL OXIDASE AND PHENOLICS IN SWEET PEAR[J];ARID ZONE RESEARCH;1995-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Zhang Yu 1 , Bai Shiqie 1 , Li Cong 2 , Li Daxu 1 , Deng Yongchang 1 (1 Sichuan Grassland Science Academy , Xipu Chengdu 611731; 2 Institute of Animal Science, Chinese Academy of Agricultural Science , Beijing 100094);Construction of Plant Expression Vector pCAMBIAl302-zeolin-GFP and the Transient Expression[J];Chinese Agricultural Science Bulletin;2012-03
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Lu Jingmei;Li Jiandong(National Laboratory of Grassland Ecological Engineering,Northeast Normal University,Changchun,130024);SEM Observation of the Anatomical Structure of Polygonum sibricum Laxm[J];JOURNAL OF NORTHEAST NORMAL UNIVERSITY (NATURAL SCIENCE EDITION);1994-03
2 Chen Ching-cheng, Sun Yang-wen, Chang Kuo-Liang;A Preliminary Study on the Morphology and Anatomy of the Dominants of the Vegetations Distributing along the Middle and Low Drainage Basin of the Shor-ler River Based on the View Point of Ecology.[J];Journal of Lanzhou University;1961-03
3 JIANG Xiang-mei,HUANG Min-ren,WANG Ming-xiu(Laboratory of Forest Genetics and Gene Engineering Nanjing For estry University,Nanjing 210037,China);A Review on Salt and Drought Resistance Gene Engineering in Plants[J];Journal of Nanjing Forestry Unversity;2001-05
4 Tu Li-zhu (Department of Biology);Anatomical Observations on Nine Xerophytes Leaves in the West Part of the Inner Mongolia[J];Acta Scientiarum Naturalium Universitatis Neimongol;1982-04
5 LU Qing (Department of biology,Medical college of Chinese People's Armed Polices Forces,Tianjin 300162, China);Development of the molecular biological studies of salt-tolerant in plants[J];Joural of Biology;2000-04
6 Wang Wei-yi (Northwest Plateau Instttute of Biology,Academia Sinica);OBSERVATIONS ON THE CAPACITY OF DROUGHT RESISTANCE OF SOPHORA MOOCROFTIANA (WALL.) BENTH.EX BAKER[J];植物学报(英文版);1980-03
7 Liu Jia yao, Yi Yan jun and Zhao Ke fu (Department of Biology, Shandong Teachers’University, Jinan 250014);EFFECTS OF SALINITY ON ION CONTENTS, BETAINE LEVEL AND BETAINE ALDEHYDE DEHYDROGENASE ACTIVITY IN SEEPWEED (SUAEDA SALSA) SEEDLINGS[J];Acta Botanica Sinica;1994-08
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