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《Plant Physiology Communications》 2007-01
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Cloning and Sequence Analysis of cDNA of Glyceraldehyde-3-phosphate Dehydrogenase Gene from Polygonum sibiricum Laxm.

LI Xiao-Ze, LIU Guan-Jun, YANG Chuan-Ping* The Provincial Key Laboratory of Forest Genetics and Breeding, The Laboratory of Forest Genetics and Breeding and Biotechnol-ogy of Ministry of Education, Northeast Forestry University, Harbin 150040, China  
The primers which were used to amplify the full length cDNA of glyceraldehyde-3-phosphate dehy-drogenase gene (GAPDH) were designed according to the expressed sequence tag (EST) sequence of GAPDH gene acquired from suppression subtractive hybridization library of the stem of Polygonum sibiricum under the stress of NaHCO3. The full length cDNA sequence of GAPDH gene was obtained by using rapid amplification of cDNA ends (RACE) technology. The results showed that the cDNA of GAPDH gene was 1 331 bp, encoding 337 amino acid residues, and deduced nucleic acid and amino acid sequence possessed high identity with the ones from the cytoplasms of other higher plants. Among these genes, the most conserved one even share 96% in their nucleic acid sequence. The results from biofunctional analyses with GAPDH yeast (Saccharomyces cerevisiae, INVSC1) transformants showed that GAPDH yeast transformants had significantly higher resis-tance to different salt stresses. INVSC1 (pYES2-GAPDH) had more salt-resistance ability than INVSC1 (pYES2) and the former survival rate was higher than that of the later under the stress of 10% NaHCO3 and 4 mol·L-1 NaCl. This indicated that the high ability of salt-tolerance of INVSC1 (pYES2-GAPDH) might be related to the expression of pYES2 gene. The GAPDH was accepted by GenBank which accession number is DQ922680.
【Fund】: 国家重大基础研究发展规划“973”项目(G1999016003);; 黑龙江省重点攻关项目(GB06B303)。
【CateGory Index】: Q943.2
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