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《Plant Physiology Journal》 2018-04
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Changes of pollen morphology, storage conditions and protective enzyme activities of Viola variegata

GUO Ying-Zi;WANG Jia-Zhe;JIA Wen-Qing;LIU Hui-Chao;HE Song-Lin;LI Ji-Yuan;WANG Yan-Li;DU Xiao-Hua;MU Jin-Yan;ZHU Xiao-Pei;College of Horticulture Landscape Architecture, Henan Institute of Science and Technology;Institute of Subtropical Forestry, Chinese Academy of Forestry;  
The pollen morphology of Viola variegata was investigated under scanning electronic microscope(SEM). The fresh pollen was took as materials to study the pollen viability by in vitro, and the effects of different storage temperatures and time on the pollen viability, superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) activities were investigated. The results showed that V. variegata had a special classification status in the genus Viola, and the most suitable medium for pollen germination was 280 g·L~(-1) sucrose+250 mg·L~(-1) boric acid+50 mg·L~(-1) GA_3+200 mg·L~(-1) Ca(NO_3)_2. The best storage temperature for pollen was –80°C. After 360 d storage of –80°C, the germination rate still reached 40.50%, while the three kinds of enzymes activities were high, the antioxidant capacity and aging ability of the pollen kept stronge. During the storage, the germination rate of pollen decreased significantly at room temperature and 4°C when the activities of SOD, POD and CAT reached a high peak. At different storage temperatures, the effects of the three protective enzymes were different. CAT was a sensitive protection enzymes at room temperature; POD was a sensitive protective enzyme and SOD was a non-sensitive protective enzyme at 4°C; and POD was a subzero hypothermia sensitive protective enzyme at –20 and –80°C. The pollen germination rate was extremely significant positively correlated with SOD.
【Fund】: 河南省基础与前沿项目(162300410157);; 河南省高等学校重点科研项目(17A21005);; 国际合作领域--科技攻关(172102410053);; 国家十二五科技支撑项目(2012BAD01B07);; 河南省产学研(152107000028);; 大学生“百农英才”创新项目(BNYC2016-2-19)~~
【CateGory Index】: S682.19
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