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《Plant Physiology Journal》 2018-04
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Tissue culture and rapid propagation of Dendrobium kingianum Bidwill

MO Yuan-Qi;ZHENG Feng;FANG Lin;LI Lin;JIANG Nan;WU Kun-Lin;ZENG Song-Jun;Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences;University of Chinese Academy of Sciences;Dongguan Agricultural Science Research Center;Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences;  
The tissue culture and rapid propagation system of Dendrobium kingianum Bidwill was established by using the pseudobulbs as explants. The results showed that the success rate of explants disinfection reached 92.5% with proper disinfection methods. MS supplemented with 5 mg·L~(-1) 6-BA was suitable for adventitious bud induction and proliferation for previous generations. The most suitable medium for the multiplication was 0.5 mg·L~(-1) NAA and 2.0 mg·L~(-1) KT and the multiplication rate was 6.96. MS medium supplemented with 0.25 mg·L~(-1) 2,4-D was the most appropriate for callus induction and the callus induction rate was 80%. MS with 1.0 mg·L~(-1) 6-BA was most suitable for callus differentiation and 381.88 buds adventitious buds were induced for 1.0 g callus. MS supplemented with 2.0 mg·L~(-1) IBA, 1 g·L~(-1) activated carbon and 10% banana homogenate was suitable for the rooting and growth of cluster buds. On the mixture substrate of orchid stone: peat soil=1:1(V:V), the cultured plantlets grew fast and the survival rate was 100%, which suggested that the substrate was suitable for transplanting in the commercial production.
【Fund】: 国家重点研发计划项目(2016YFC0503104);; 东莞市社会科技发展项目(2016108101001);; 中国科学院战略生物资源服务网络计划植物种质资源创新项目(ZSZC-008);; 广东省现代农业产业技术体系花卉创新团队项目(2016LM1142)~~
【CateGory Index】: S682.31
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