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《Plant Physiology Journal》 2018-04
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Early stage observation and conditional optimization for proliferation of resistant Pinus densiflora somatic embryogenesis

PAN Jun;WU Xiao-Qin;XU Jian-Xiu;YE Jian-Ren;Collaborative Innovation Center of Southern Modern Forestry, Forestry Department, Nanjing Forestry University;  
In order to optimize the conditions of somatic embryogenesis of resistant Pinus densiflora, this study investigated the zygotic embryo distribution in the seed chamber and the changes of embryonic suspensor structure by qualitative observation with stereoscopic mirror; and investigated the change of embryo and suspensor ratio in different hormone condition for quantitative observation by Image-Pro Plus software, aiming at the nutritional needs at this stage. The results showed cleaved polyembryony were more easily differentiate into embryogenic cells. The change of the original culture environment would separate embryo from suspensor and the embryo would accumulate more material. The medium(0.1 mg·L~(-1) 2,4-D+1 mg·L~(-1) NAA+0.5 mg·L~(-1) 6-BA) would improve the proliferatin speed. It improved the mechanism of improve proliferation in resistant P. dnsiflora was the increase of the embryo and suspensor ratio.
【Fund】: 江苏省科技支撑计划(BE2014405);; 江苏高校优势学科建设工程资助项目(PAPD)~~
【CateGory Index】: S791.245
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