Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Plant Physiology Journal》 2018-12
Add to Favorite Get Latest Update

Virus-free culture and virus detection of stem tip of Pinellia ternata

WANG Bao-Xia;QI Yong-Hong;XIAO Ya-Yin;CUI Li-Yan;WANG De-Fu;NIU Yan-Bing;College of Life Sciences, Shanxi Agricultural University;Fruitry Station of Shanxi Province;  
Absrtact: The stem tips were taken as explants to study the induction effects of different hormone concentrations on virus-free stem tips of Pinellia ternata seedlings. On the basis of single factor experiment, L9(32) orthogonal test of 6-benzylamino adenine(6-BA) and 2,4-dichlorophenoxyacetic acid(2,4-D) with three optimal concentrations was conducted to screen the optimal medium of callus induction, followed by molecular detection of the virus condition of virus-free seedlings. The results showed that the callus induction rate was the highest when the concentration of 6-BA was 1.5 mg·L~(-1) and 2,4-D was 1.8 mg·L~(-1). The optimum medium for callus induction was MS+1.5 mg·L~(-1) 6-BA+1.8 mg·L~(-1) 2,4-D+7 g·L~(-1) agar+30 g·L~(-1) sugar, which could induce the seedlings at one step. After reverse transcription-polymerase chain reaction(RT-PCR) and dot-enzymelinked immunosorbent assay(DOT-ELISA) detection, the virus-free rate of Tobacco mosaic virus(TMV), Cucumber mosaic virus(CMV) and Soybean mosaic virus(SMV) reached 100%.
【Fund】: 国家自然科学基金(31601612)~~
【CateGory Index】: S567.239
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 XIE Xuan;XU Ke;XIE Min-Xin;ZHU Yuan-Di;College of Agriculture and Biotechnology, China Agriculture University;;Optimization of Rapid Micropropagation System of Apple Meristem-Tip Culture[J];植物生理学报;2015-12
2 ZHANG Yan-Ling;TANG Cheng-Ying;HE Fu;WU Xiu-Hua;TANG Shao-Hu;Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Southwest University;School of Life Science, Southwest University;;Tissue Culture and Rapid Propagation of Rare and Endangered Ficus beipeiensis[J];植物生理学报;2015-04
3 LIN Qiu-yue;ZENG Wan-yong;LI Tian-pei;LI Rui;CHEN Ping;LI Jin-hua;School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University;;Screening of Optimal Medium for Callus Inducing and Redifferentiation of Pinellia ternata(Thunb.)Breit.[J];湖北农业科学;2014-13
4 CHENG Xia-ying, YANG Zong-qi (College of Life Sciences,Zhejiang Sci-Tech University,Hangzhou,Zhejiang 310018,China);Optimization Study of Callus Induction on Pinellia ternata[J];天津农业科学;2013-09
5 HUANG Fang1,LI Bo1,TIAN Shan1,MENG Jia-jia1,RAN De-bing1,LIU Yue-feng2,LI Shang-wei2(1.College of Agriculture,Guizhou University,Guiyang,Guizhou 550025;2.Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region,Guiyang,Guizhou 550025,China);Research Progress in Pharmacological Actions and Insecticidal Activities of Rhizoma arisaematis and Rhizome pinellia[J];贵州农业科学;2011-10
6 NIU Yan-bing,YAO Min,WANG De-fu,WANG Gui-jun,GONG Qiang(College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China);Identification of the pathogenic virus on Ailanthus altissima[J];植物病理学报;2011-04
7 PENG Xiang-qian,ZHANG Wen-hui(College of Life Science,Liaocheng University,Liaocheng,Shandong 252059,China);TECHNOLOGY SYSTEM FOR STEM-APEX MERISTEM CULTURE AND RAPID PROGAGATION OF PINELLIA TERNATA(THUNB.) BREIT[J];井冈山大学学报(自然科学版);2011-02
8 PAN Wei-cang,LIU Ju-ying(1.College of Life Sciences and Chemistry,Tianshui Normal University,Tianshui,Gansu 741001,China;2.Yongdeng No.2 Middle School,Yongdeng,Gansu 730302,China);Induction of Callus and Regeration Pinellia ternate in Tianshu[J];北方园艺;2009-11
9 ZHOU Yi-jun~(1,2),ZHOU Sheng-chuang~1,LIU Yu~1,ZHANG Gen-fa~2(1.College of Life Science,Beijing Normal University,Beijing 100081,China;2.College of Life and Environmental Science,Central University for Nationalities,Beijing 100875,China);Influences of Plant Growth Regulations on Induction and Differentiation of Plant Callus[J];中央民族大学学报(自然科学版);2007-01
10 LIU Wenhong,CHEN Jishuang,LI Yongwei (College of Life Science,Zhejiang University,Hangzhou 310029,China);Molecular identification of Araceae-infecting viruses and their ecology.[J];应用生态学报;2004-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HU Tiantian;WANG Yali;YANG Xiulian;WANG Lianggui;College of Landscape Architecture,Nanjing Forestry University;;Study on Pedicels and Leaves Callus Induction of Osmanthus fragrans[J];北方园艺;2015-19
2 WANG Hua-yu;Nanyang City Forestry Research Institute;;Study on Application Techniques of Plant Growth Regulator Foliar Treasure[J];园艺与种苗;2015-10
3 JIANG Yanfengyang;LIN Chao;ZHAN Ruoting;MA Xinye;Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Key Laboratory of Chinese Medicine Resources of Ministry of Education;;Study on Callus Induction in Explant of Aquiliaria sinensis Root[J];广州中医药大学学报;2015-04
4 WANG Li-xue;FAN Zhu-ping;ZHANG Yan-ni;College of Landscape, Northeast Forestry University;;Overview of Research on the Tissue Culture of Fittonia verschaffeltii[J];天津农业科学;2015-06
5 SHENG Jia-jing;ZHONG Lin;YANG Chao-zhu;HU Kai;WANG Da-ping;WU Jin-ping;HU Zhong-li;DIAO Ying;College of Forestry and Life and Sciences, Chongqin University of Arts and Sciences;College of Life Sciences, Wuhan University;Institute of Konjac, Enshi Academy of Agricultural Sciences;Institute of Economic Crop, Hubei Academy of Agricultural Science;;Detection of Dasheen Mosaic Virus in Amorphophallus konjac[J];湖北农业科学;2015-10
6 SHENG Jia-jing;ZHONG Lin;YANG Chao-zhu;ZHANG Shao-bin;XIE Ji-rong;WU Jin-ping;HU Zhong-li;DIAO Ying;College of Forestry and Life Science,Chongqing University of Arts and Sciences;College of Life Science,Wuhan University;Institute of Konjac Research,Enshi Prefecture Academy of Agricultural Sciences;Institute of Economic Crop, Hubei Academy of Agricultural Sciences;;Method for Detecting Cucumber Mosaic Virus in Konjac on Molecular Level[J];湖北农业科学;2015-09
7 Du Jiang;Cheng Yuanyuan;Niu Erbo;Niu Yanbing;College of Life Science,Shanxi Agricultural University;;Sequence analysis of CP gene of Cucumber green mottle mosaic virus isolate infecting watermelon from Shanxi Province[J];植物保护;2015-02
8 NIU Yanbing;SHI Xiaoli;ZHANG Ximei;ZHAO Huiqi;ZHAO Baojia;College of Life Sciences,Shanxi Agricultural University;;Molecular Identification and Sequence Analysis of Broad Bean Wilt Virus 2 Isolates from Atractylodes Macrocephala Koidz[J];病毒学报;2015-01
9 CHEN Jin-fang;HE Xiao-bing;ZHANG Li;DING Yan-fen;College of landscape architecture,Nanjing Forestry University;;Research Progress on the Factors Affecting Callus Differentiation of Ornamental Trees[J];福建林业科技;2014-04
10 ;Clinical observation on treating acute bronchitis in children with Xiao'er Qingfei particle[J];中医临床研究;2014-35
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Miao-miao;MA Xiao-yue;ZHANG Xue-ying;XU Ji-zhong;College of Horticulture,Agricultural University of Hebei;;Study on Rapid Micropropagation Technique of Shoot Tip Culture of Cold-Resistant Apple Dwarf Rootstock 71-3-150[J];西部林业科学;2014-06
2 Hu Guojun;Dong Yafeng;Zhang Zunping;Fan Xudong;Ren Fang;Zhu Hongjuan;National Center for Eliminating Viruses from Deciduous Fruit Tree,Research Institute of Pomology,Chinese Academy of Agricultural Sciences;;Research progress in virus elimination techniques for apple trees[J];植物保护;2014-06
3 SUN Hong-Yan;SUN Qing-Rong;LI Guo-Tian;ZHANG Qiong;LI Qin;Shandong Institute of Pomology;;Tissue Culture and Shoot Regeneration from in Vitro Leaf Explants of Apple Dwarfing Rootstock Cultivar ‘JM7'[J];植物生理学报;2014-06
4 CHENG Xia-ying, YANG Zong-qi (College of Life Sciences,Zhejiang Sci-Tech University,Hangzhou,Zhejiang 310018,China);Optimization Study of Callus Induction on Pinellia ternata[J];天津农业科学;2013-09
5 WU Xiu-Hua, ZHANG Yan-Ling, ZHOU Yue, LUO Ke-Ming, TANG Shao-Hu * Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China;Establishment of High Frequency and Direct Regeneration System from Leaf of ‘Hayward’ Kiwifruit [Actinidia deliciosa (A. Chev. ) C. F. Liang et A. R. Ferguson][J];植物生理学报;2013-08
6 ZENG Wan-yong,LI Jin-hua,WANG Zhi,YANG Yu-wen,HU Qian(School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China);Research on the condition of aseptic germination and plantlet rapid propagation of dendrobium officinale kimura et migo[J];武汉工业学院学报;2012-03
7 ZHAO Jing,SHUAI Ming-rong, ZHAO Yu-fei,LI Xian-yuan,LI Ming-yang School of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716,China;Tissue Culture of Lilium rosthornii Diels and a Rapid Propagation System for It[J];西南师范大学学报(自然科学版);2012-06
8 CUI Mei1,JIAO Qi-qing2,CHEN Xue-sen1,CHEN Xiao-liu1(1.College of Horticulture Science and Engineering,Shandong Agricultural University/ State Key Laboratory of Crop Biology, Taian 271018,China;2.Shandong Institute of Pomology,Taian 271000,China);Induction Culture of Callus from Apple Leaves[J];山东农业科学;2012-03
9 Wen LI,Yeming XIE,Cuiping LUO,Jinhua LI,Wanyong ZENGSchool of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Study on Rapid Propagation Medium of Atractylodes Lances by Orthogonal Design[J];Agricultural Science & Technology;2012-01
10 YANG Guo-tai,LI Liang,ZHANG Dong-min,ZHONG Feng-lin;Research of Controlling the Pollution of Endophyticab Cteria in the Process of Tissue Culture[J];中国园艺文摘;2011-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved