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《植物学报(英文版)》 1984-01
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Xu Zhi-hong (Shanghai Institute of Plant Physiology,Academia Sinica)Huang Bin (Institute of Genetics,Academia siniea)  
The effects of anther tissues were studied systematically on microspores forming multicellular units and furthermore on pollen callus formation in the anther culture of Hordeum vulgare(cv.Sabarlis).Anther productivity was found to be greatly enhanced by use of medium previously conditioned by anthers.In 15 experiments observed,anthers pro- duced 26 times on average more calli in the conditioned medium than in control,in a few cases,even more than 80 times more calli formed.According to this,the authors supposed that cultured anthers released some components,anther factor(s)(AF),which is important to androgenesis in the culture.To achieve high yields of callus,culture was restricted to an- thers which had been subpected to cold pretreatment.The temperature stress could not be replaced by the AF.However,for conditioning medium,anthers at binuclear stage were found to be more effective than the test anthers either with or without the pretreatment. Anthers from other 8 barley varieties were also effective for conditioning,as the difference of anther productivity still existed in the culture with conditioned medium between various genotypes tested.Anther response and callus yield were increased in both the culture of an- thers at mid and late-uninuclear stage by use of conditioned medium. AF interacted synergistically with m-inositol.Cytological observation showed that AF increased apparently the formation of MPGs,while m-inositol mainly stimulated callus for- mation from MPGs.To some extent,the effect of exogenous hormone(s)could be replaced by AF.The anther response and pollen callus yield could be much enhanced by increa- sing anther inoculation density,which also raised the AF level in the culture. Thus,by use of the temperature stress prior to anther culture and culture of test an- thers in conditioned medium with m-inositol,or at higher inoculation density,a very high production of pollen callus could be obtained in barley anther culture.For meeting the more specialized requirements of less responsive species or genopypes,the principles given here may be provide some basic information.
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Chinese Journal Full-text Database 2 Hits
1 TENG Hai-tao1,LU Shou-ping2,ZHAO Bang-qing3,KONG Shao-hua4,LI Yong1(1.Maize Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China;2.Shandong Academy of Agricultural Science,Ji'nan 250100,China;3.Zhangjialou Town Agricultural Service Center of Jiaonan Country in Shandong Province,Jiaonan 266406,China;4.Linyi Country Agricultural Bureau,Linyi 251500,China);Pretreatment of Plant Anther Culture and its Physiological and Biochemical Changes[J];Journal of Hebei Agricultural Sciences;2008-10
2 XUE Jian_Ping ZHANG Ai_Min (Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000);Studies on Changes of Anther Wall Tissues of Different Genotypes of Wheat during Anther Culture[J];Chinese Bulletin of Botany;2002-03
Chinese Journal Full-text Database 10 Hits
1 Wang Shuqiang; Wang Shanmin; Liu Yuhong (Commission for Integrated Survey of Natural Resources,CAS Beijing 100010);A Study on Tissue Culture and Plant Regeneration of Psathyrostachys perennis Keng[J];ACTA AGRESTIA SINICA;1997-03
2 Bai Keyu; Wang Pei ;Han Jianguo(Institute of Grassland Science, China Agricultural University, Beijing 100094)Sun Ruichen(Chengde Yuershan Farm, Yuershan 068359);Nitrogen Distribution at Grass-Soil System and Growing Dynamic on Russian Wildryegrass Pasture as Influenced by Grazing[J];ACTA AGRESTIA SINICA;1999-01
3 ZHANG Hui1,WEI Jian-hua~2,HUO Xiu-wen~1,XU Chun-bo~1,MI Fu-gui~(1*),YUN Jin-feng~(1*) (1.Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010018, China; 2.Beijing Center of Agro-Biotechnology, Beijing 100089, China);A Study on the Induction of Embryogenic Callus and Plant Regeneration from Mature Embryos of Agropyron cristatum× Agropyron desertorum cv. Mengnong[J];Acta Agrestia Sinica;2005-01
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5 Wang Feng; Wang Pei; Cheng Yurong; He Ping(Institute of Cereal and Oil Crops, Hebei Academy of Agricultural Sciences, Shijiazhuang. 050031);Study on Factors Affecting Submerged Culture of Anthers in Wheat[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1994-02
6 Shi Yongjie Sun Xiaoping Liu Xiaoqiang (Lanzhou Institute of Animal Science and Veterinary medicine, CAAS, Lanzhou 730050);TISSUE CULTURE OF YOUNG SPIKELETS OF BOTHRIOCHLOA ISCHAEMUM[J];PRATACULTURAL SCIENCE;1998-06
7 Liao Xiangru1 Du Jianfang1 Wang Junxia2 Wang Junli1 Song Luhua1 Chen Piling2 Zhu Baocheng2( 1 Research Center of Biotechnology,Hebei University, Baoding 071002; 2 Department of Biology,Hebei University, Baoding 071002);Effect of Seed Pretreatment on Callus Formation of Wheat Mature Embryo[J];JOURNAL OF HEBEI UNIVERSITY(NATURAL SCIENCE EDITION);1999-01
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10 GAO Jun-shan~(1,2),YE Xing-guo~2,MA Chuan-xi~1 ,LIN Yi~(1)((1. Anhui Agricultural University, Hefei 230036,Anhui,China;2. Institute of Crop Breeding and Cultivation,CAAS,Beijing 100081,China);Studies on Wheat Regenation Ability by Different Tissue Culture Approach[J];Acta Laser Biology Sinica;2003-06
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 SUI Xin-xia, FAN Qing-qi, LI Gen-ying, HUANG Cheng-yan (Crop Research Institute, Sandong Academy of Agricultural Sciences, Jinan 250100 china);Review on Wheat Anther Culture[J];Acta Tritical Crops;2005-04
2 HE Yu-chi~1 GE Jie~1 TAO Cai-cheng~2 WANG He-qing~2 CAI De-tian~1 (1 College of life science,Hubei University,Wuhan Hubei 430062 China)(2 Agronomy department,Changjiang University,Jingzhou Hubei 434103 China);Analysis of Genotype Stability and Media Condition Index of Barley(Hordeum vulgare L.)Anther Culture[J];Journal of Biomathematics;2005-03
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