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《植物学报(英文版)》 1984-01
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ANTHER FACTOR(S) IN BARLEY ANTHER CULTURE

Xu Zhi-hong (Shanghai Institute of Plant Physiology,Academia Sinica)Huang Bin (Institute of Genetics,Academia siniea)  
The effects of anther tissues were studied systematically on microspores forming multicellular units and furthermore on pollen callus formation in the anther culture of Hordeum vulgare(cv.Sabarlis).Anther productivity was found to be greatly enhanced by use of medium previously conditioned by anthers.In 15 experiments observed,anthers pro- duced 26 times on average more calli in the conditioned medium than in control,in a few cases,even more than 80 times more calli formed.According to this,the authors supposed that cultured anthers released some components,anther factor(s)(AF),which is important to androgenesis in the culture.To achieve high yields of callus,culture was restricted to an- thers which had been subpected to cold pretreatment.The temperature stress could not be replaced by the AF.However,for conditioning medium,anthers at binuclear stage were found to be more effective than the test anthers either with or without the pretreatment. Anthers from other 8 barley varieties were also effective for conditioning,as the difference of anther productivity still existed in the culture with conditioned medium between various genotypes tested.Anther response and callus yield were increased in both the culture of an- thers at mid and late-uninuclear stage by use of conditioned medium. AF interacted synergistically with m-inositol.Cytological observation showed that AF increased apparently the formation of MPGs,while m-inositol mainly stimulated callus for- mation from MPGs.To some extent,the effect of exogenous hormone(s)could be replaced by AF.The anther response and pollen callus yield could be much enhanced by increa- sing anther inoculation density,which also raised the AF level in the culture. Thus,by use of the temperature stress prior to anther culture and culture of test an- thers in conditioned medium with m-inositol,or at higher inoculation density,a very high production of pollen callus could be obtained in barley anther culture.For meeting the more specialized requirements of less responsive species or genopypes,the principles given here may be provide some basic information.
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【References】
Chinese Journal Full-text Database 2 Hits
1 TENG Hai-tao1,LU Shou-ping2,ZHAO Bang-qing3,KONG Shao-hua4,LI Yong1(1.Maize Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China;2.Shandong Academy of Agricultural Science,Ji'nan 250100,China;3.Zhangjialou Town Agricultural Service Center of Jiaonan Country in Shandong Province,Jiaonan 266406,China;4.Linyi Country Agricultural Bureau,Linyi 251500,China);Pretreatment of Plant Anther Culture and its Physiological and Biochemical Changes[J];Journal of Hebei Agricultural Sciences;2008-10
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【Co-references】
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7 Liao Xiangru1 Du Jianfang1 Wang Junxia2 Wang Junli1 Song Luhua1 Chen Piling2 Zhu Baocheng2( 1 Research Center of Biotechnology,Hebei University, Baoding 071002; 2 Department of Biology,Hebei University, Baoding 071002);Effect of Seed Pretreatment on Callus Formation of Wheat Mature Embryo[J];JOURNAL OF HEBEI UNIVERSITY(NATURAL SCIENCE EDITION);1999-01
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【Secondary References】
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1 SUI Xin-xia, FAN Qing-qi, LI Gen-ying, HUANG Cheng-yan (Crop Research Institute, Sandong Academy of Agricultural Sciences, Jinan 250100 china);Review on Wheat Anther Culture[J];Acta Tritical Crops;2005-04
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