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《植物学报(英文版)》 1984-01
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FURTHER STUDIES ON POLLEN WALL GLYCOPROTEINS IN CUCURBITA PEPO L.

Yang Zhong-han Zhu Guang-lian Cao Zong-xun(Tsao,T.H.) (Department of Biology,Peking University)  
Samples of pollen wall protein of Cucurbita pepo were prepared as repor- ted in previous paper.Gas chromoatographic analyses showed that the carbohydrate fraction of the pollen wall glycoprotein contained 20.4% rhamnose,15.3% fucose,11% mannose, 11% galactose,31% glucose,4% arabinose and traces of xylose.The glycoproteins were further purified by Con.A affinity chromatography.Isoelectric focussing electrophoresis of the purified sample showed 3 PAS-positive bands,with respective PI 5.2,6.0 and 6.3.The glycoprotein samples were subjected to hydrolysis with 6N HC1.After hydrolysis,the mix- ture was analyzed for amino acid composition with Backman 121-MB automatic amino acid analyzer,Results show the amino acid composition of the 3 glycoprotein was very simi- lar,They all have glycine,glutamic acid and serine as their major component(these three amino acids constitute 50—60% of the total amino acids);and they all contain very small amount of methionine,phenylalanine,isoleucine and tyrosine.The lysine content of each glycoprotein is consistent with its respective PI,the glycoprotein which contains more ly- sine has higher PI.
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【References】
Chinese Journal Full-text Database 1 Hits
1 LI Ye-feng,XIE Hui-hui,LIU Xiao-hong,WANG Qun,LU Xiao-ping*(Gold Mantis School of Architecture and Urban Environment,Soochow University,Suzhou 215123,China);Surface Proteins Analyzed by SDS-PAGE in the Pharbitis nil(Linn.) Choisy Pollen[J];Journal of Henan Agricultural Sciences;2010-01
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 CHENG Ping et al(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101);Molecular Study on the Plant Self-incompatibility[J];Journal of Anhui Agricultural Sciences;2008-32
2 QIN Xin-min,LV Jian-zhen,LI Hui-min,XUE Miao-nan,YANG Ji-hua (College of Life Science,Guangxi Normal University,Guilin 541004,China);THE TWO-DIMENSIONAL ELECTROPHORESIS OF PROTEIN FROM POLLEN WALL BETWEEN CITRUS GRANDIS VAR. SHATINYU HORT.AND CITRUS GRANDIS[J];Journal of Guangxi Normal University(Natural Science);2004-04
3 WANG Ai-yun 1,LI Xun1,HU Da-you2(1.College of Agronomy,HNAU,Changsha 410128,China;2.Yiyang Vocational and Technological College,Yiyang,Hunan 413000,China);Pollen-Stigma Interaction Between Orychophragmus violaceus and Brassica Species[J];Journal of Hunan Agricultural University(Natural Sciences);2006-03
4 YUAN Kun(Nanjing Forestry University,Nanjing 210037,China);Brief review on pollenstigma recognition in plants[J];Journal of Jiangsu Forestry Science & Technology;2004-03
5 LI Xin-Lei1,2,CHEN Fa-Di1(1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang,Zhejiang 311400,China);Self-incompatibility in Dendranthema morifolium[J];Journal of Wuhan Botanical Research;2007-06
6 Yang Zhonghan Cao Zongxun (Biology Department, Beijing University);Physicochemical Properties of Pollen-wall Protein of Cucurbita pepo and Luff a cylindrica and Its Possible Role in“Recognition”Reaction[J];Acta Genetica Sinica;1981-01
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