Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《植物学报(英文版)》 1989-01
Add to Favorite Get Latest Update


Wang Guang-yuan Xia Zhen-an(Hsia Chen-au) Chi Jing-fen (Shanghai Institute ot Plant Physiology,Academia Sinica)  
Cotyledon protoplasts of tomato(Lycopersicon esculentum L.)isolated from 2—3 week- grown seedlings were cultured in MS liquid medium(2,4-D 1,6 BA 0.1 mg/l)and fresh medium added subsequently.After 6 weeks culture,the cell clusters were transferred to semisolid me- dium(the additive same as in liquid medium,agar 0.3%).When the calli grew to 0.5 cm in diameter,transfer them to MS medimn(6BA 2,IAA 0.2 mg/l)for differentiation.The re- generated plants were obtained.After comparing different culture methods,tomato protop- lasts grew better in double layers than in agar plate and hanging drops.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 8 Hits
1 FENG Xian-hong LI Jian YANG Kai-jun(Sichuan Ganzi Agricultural Research Institute,Kangding Sichuan 626000);Research on Browing in Plant Tissue Culture[J];;2009-23
2 WANG Bao-ning1,ZHANG Xian2,SONG Jun-yang1(1.College of Forestry,Northwest Agricultural and FoRestry University,Yangling,Shaanxi 712100;2.College of Forestry,Northwest Agricultural and FoRestry University,Yangling,Shaanxi 712100);Studies on Browning of Regenerated System of Wild Cymbidium goeringii in QinLing[J];Northern Horticulture;2011-05
3 Shu Wenhua Geng huazhu (Animal science institute, Chinese academy of agricultural science, Beijing, 100094) Yian lunxing (Grassland department of Animal science college of Inner Mongolia Autonomous Region, Tongliao, 028000) Xu yuqing (Sino—Czechoslovak friendship farm, Hebei, 067108);TISSUE CULTURE AND PLANT REGENERATION FROM CALLUS OF COTYLEDON OF ALFALFA (MEDICAGO SATIVA L.)[J];Pratacultural Science;1993-03
4 PANG Yong(Lanzhou Agricultural School, Lanzhou 730020, Gansu)\;;Research Progress of Browning Phenomenon in Tissue Culture of Fruit Trees[J];Journal of Gansu Forestry Science and Technology;2004-01
6 SI Jun,LI Cheng-qiong (Department of horticulture,Southwest Angricultural University,Chongqing,400716,China);Research progress and prospect on anther culture of tomato[J];Joural of Biology;2002-01
7 Zhang Xingguo Chen Jinfeng Zhang Shenglin Su Chenggang Liu Peiying (Horticultural Department,Southwest Agricultural Univ.);STUDIES ON THE FACTORS AFFECTING THE ISOLATION AND CULTURE OF PROTOPLASTS OF AMORPHOPHALLUS[J];Journal of Southwest Agricultural University;1992-01
8 Zhou Junhui, Zhou Jiarong, Zeng Haosen, Wang Guobing, and Zhu Zhanping ( Department of Horticulture, Zhongkai Agrotechnical College, Guangzhou 510225; Department of Element, Zhongkai Agrotechnical College, Guangzhou 510225);Advance of Studies on Browning and Antibrowning Techniques in the Tissue Culture of Horticultural Plants[J];Acta Horticulturae Sinica;2000-S1
Chinese Journal Full-text Database 2 Hits
2 ZHU Danhua~1 and M. KOORNNEEF~2 1 Crop Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 2 Department of Genetics, Wageningen Agricultural University, Wageningen 6703 HA, The Netherlands;Association of Peroxidase with Growth and Regeneration of Protoplast-Derived Callus in Tomato[J];Acta Agriculturae Zhejiangensis;1992-03
Chinese Journal Full-text Database 10 Hits
1 Wang Xiufeng (Rice Research Institute,Anhui Academy of Agricultural Sciences, Hefei 230031);Study on the Browning Problem in Plant Tissue Culture[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1999-04
2 YANG Lu-jun et al(Zhengzhou Institute of Science from Agriculture and Forest in Henan Province,Zhengzhou,Henan 450005);Study on Establishment of Regeneration System of Cymbidium[J];Journal of Anhui Agricultural Sciences;2008-32
3 Chen Jinyong(Beijing Botanical Garden,Beijing,100093,P.R.China)Cheng Jinshui(College of Landscape Architecture ,Beijing For.Univ.,100083,P.R.China)Zhu Ying(Vocational School of Gucheng,Beijing,100043,P.R.China);The Morphogenesis of Chinese Orchid Under in vitro Seed Germination[J];Journal of Beijing Forestry University;1998-01
4 Chen Jinyong(Beijing Botanical Garden,Beijing,100093,P.R.China)Cheng Jinshui(College of Landscape Architecture,Beijing For. Univ.,100083,P.R.China)Zhu Ying(Vocational School of Gucheng,Beijing,100043,P.R.China);Callus Development of Chinese Orchid Under in vitro Seed Germination[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1998-04
5 Luo Xiaofang Tian Yanting Yao Hongjun (College of Plant Sciences, Beijing For. Univ., 100083,P.R.China);Polyphenol Oxidase Activities and Phenol Contents in Tissue Culture[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-01
6 Jin Hong ;Li Gao; Wu Yunfei(Department of Grassland Science, Inner Mongolia Institute of Agriculture and Animal Husbandry, Huhhot 010018);Studies on the Histology of Organogenesis in Isolated Culture of Mature Embryo of Melilotoides ruthenicus(L.) Sojak cv.‘Zhili'[J];ACTA AGRESTIA SINICA;1997-04
7 Yang Yansheng(Institute of Soil Science,the Chinese Academy of Sciences,Nanjing,210008)Liu Baigen; Sha Jishi(Ningdu County Water-Soil COnservation Station,Ningdu County,342800);STUDY ON THE CULTIVATION OF LESPEDEZA BlCOLOR FOR WATER-SOLL RESOURCES RESTORATION[J];PESOURCES AND ENUIRONMENT IN THE YANGTZA VALLEY;1994-04
8 SONG Xiao-xia ZHANG Xing-guo MU Sheng-yu WANG Hui-xia (Southwest Uninersity,Chongqing 400715);Agrobacterium Mediated Short Chain Fructooligosaccharide Gene Transfer of Tomato(Lycopersicon Esculentum)[J];Journal of Chongqing University of Science and Technology(Natural Sciences Edition);2007-01
9 Zhang HanshuangLi JunlanGao PengWang Haibo (Cotton Insititute,HAAFS, Shijiazhuang 050051);Embryogenesis and Plant Regeneration from SomaticCells Culture of Glandless Upland Cotton[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-01
10 LIANG Mei- xia1, 2,XU Xiang-yang1,LI Jing- fu1 ( 1. Horticultural College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC;2. Horticultural Department, Laiyang Agricultural College, Qingdao Shandong 265200, PRC );Effect of antibiotics on the induction and differentiations of tomato callus[J];Journal of Northeast Agricultural University;2005-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LI Yue-qin et al(College of Life Sciences,Sichuan University,Chengdu,Sichuan 610065);Study on the Rare and Endangered Plant Davidia involucrate Baill Tissule Culture[J];Journal of Anhui Agricultural Sciences;2007-18
2 LU Cheng-xiang et al (College of Forestry,Gansu Agricultural University,Lanzhou,Gansu 730070);Preliminary Discussion on Tissue Culture Technology for the Stem-tip in the Rare and Protected Plant Dendrobenthamia japonica var.emeinsis[J];Journal of Anhui Agricultural Sciences;2008-04
3 CAI Xiao-dong et al (College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025);Study on Poinsettia Callus Induction,Multification and Establishment of Suspension Cells[J];Journal of Anhui Agricultural Sciences;2008-26
4 WEI Peng-xiao et al(College of Agriculture,Guangxi University,Nanning,Guangxi 530005);Study on Anther Culture for Inducting Callus of Thermo-sensitive Genic Male Sterile Line of Tomato[J];Journal of Anhui Agricultural Sciences;2009-19
5 GONG Xiao-jie(School of Life Science,Lanzhou University,Lanzhou,Gansu 730000);Inhibition Effect of Several Antioxidants on Browning of the Potato Callus Culture[J];Journal of Anhui Agricultural Sciences;2009-22
6 WANG Fei et al(Department of Environmental Engineering,Xuzhou Engineering College,Xuzhou,Jiangsu 221008);Research Progress on the Tissue Culture of Tulip[J];Journal of Anhui Agricultural Sciences;2010-16
7 WEI Peng-xiao et al(College of Agriculture,Guangxi University,Nanning,Guangxi 530005);Study on the Pollen Development in Thermo-sensitive Genic Male Sterile Line of Tomato and Its Effect on Anther Callus Induction[J];Journal of Anhui Agricultural Sciences;2010-17
8 CHEN Jia et al(Jinling College,Nanjing University,Nanjing,Jiangsu 210089);Tissue Culture of Lespedeza cyrtobotrya[J];Journal of Anhui Agricultural Sciences;2011-13
9 FENG Xian-hong LI Jian YANG Kai-jun(Sichuan Ganzi Agricultural Research Institute,Kangding Sichuan 626000);Research on Browing in Plant Tissue Culture[J];;2009-23
10 XIN Jian-hua,ZHANG Yong-hua,YUAN Yu-wen(Department of Horticulture,College of Agriculture,Shihezi University,Xinjiang 832000);Study on Interrelation of Cytological Development Period of Processing Tomato Microspor and Morphology of Flower Organ[J];Northern Horticulture;2007-05
China Proceedings of conference Full-text Database 1 Hits
1 MA Wen-qing LI Qing (College of Landscape Architecture,Beijing Forestry University, National Engineering Research Center for Floriculture,Beijing 100083);Effects of Hormone on Clustered Shoots Induction and Proliferation of Cymbidium hybridum[A];[C];2009
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved