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《植物学报(英文版)》 1989-01
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PLANT REGENERATION FROM COTYLEDON PROTOPLASTS OF TOMATO(LYCOPERSICON ESCULENTUM L.)

Wang Guang-yuan Xia Zhen-an(Hsia Chen-au) Chi Jing-fen (Shanghai Institute ot Plant Physiology,Academia Sinica)  
Cotyledon protoplasts of tomato(Lycopersicon esculentum L.)isolated from 2—3 week- grown seedlings were cultured in MS liquid medium(2,4-D 1,6 BA 0.1 mg/l)and fresh medium added subsequently.After 6 weeks culture,the cell clusters were transferred to semisolid me- dium(the additive same as in liquid medium,agar 0.3%).When the calli grew to 0.5 cm in diameter,transfer them to MS medimn(6BA 2,IAA 0.2 mg/l)for differentiation.The re- generated plants were obtained.After comparing different culture methods,tomato protop- lasts grew better in double layers than in agar plate and hanging drops.
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