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《植物学报(英文版)》 1989-01
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Xing Miao Hao Shui Tong De-juan (Department of Biology,Northeast Normal University,Changchun)  
A small intranuclear body,termed spherule,mininucleolus,karyosome,nuclear body or coil- ed body,has been reported in the cell of higher plants.This intranuclear body has been suggested to represent products of transcription,to correspond to the complex of transcription,or to be related to the RNA metabolism after transcription.Opinions on its composition are diverse and the origin and functions of the body are still obscure. We studied this intranuclear body,called mininucleolus in our paper,and found that the body appeared as an ovoid or spherical structure about 0.3 to 0.5 μm in diameter.The mininu- cleolus was shown,with conventional TEM staining,to be the same electron-dense as the con- densed chromatin,but no structural connections could be found between them.The results of Bernhard's staining and the protein specific staining indicated that the mininucleolus contained both RNA and proteins which formed ribonucleoprotein(RNP)particles about 15 μm in diame- ter.These particles are very similar,in their ultrastructure and chemical composition,to the granular comoonents of the nucleolus.A careful examination revealed that the mininucleoli were formed in the granular region of the nucleolus.After they became separated from the nucleolus,these bodies were distributed throughout the nucleoplasm,suggesting that they may move from the nucleolus to the nuclear membrane.Based on these data,we suggest that the mininucleolus is a non-chromatin intranuclear structure which is formed in the nucleolus and may have a function of transporting ribosomal RNA from the nucleolus to the cytoplasm.
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Chinese Journal Full-text Database 1 Hits
1 CAO Qing-He,CHEN Jin-Feng~,GUO Jun-Yang,QIAN Chun-Tao College of Horticulture, Nanjing Agricultural University,Nanjing210095,China);Observation on Micronucleolus during Meiosis of Pollen Mother Cell in Cucumber[J];Journal of Wuhan Botanical Research;2005-01
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1 SHI Yan-xia, LI Bao-ju, LIU Xue-min(Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081 PRC;Northeast Agricultural University, Harbin 150030 PRC;The study of cucumber downy mildew[J];Journal of Northeast Agricultural University;2002-04
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